Probiotic Bacteria Reduce Salmonella Typhimurium Intestinal Colonization by Competing for Iron
| Autor: | Heidi Contreras, Stephen J. Libby, Roxanna J. Ochoa, Elisa Deriu, Ferric C. Fang, Manuela Raffatellu, Milad Pezeshki, Robert Edwards, Janet Z. Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Salmonella typhimurium
Salmonella Siderophore Cancer Research Iron medicine.disease_cause Microbiology Article law.invention 03 medical and health sciences Probiotic Mice law Virology Immunology and Microbiology(all) medicine Escherichia coli Animals Humans Colonization Colitis Intestinal Mucosa Molecular Biology 030304 developmental biology 0303 health sciences biology 030306 microbiology Probiotics biology.organism_classification medicine.disease Mice Inbred C57BL Intestines nervous system Salmonella enterica Salmonella Infections Parasitology Female Bacteria |
| Popis: | SummaryHost inflammation alters the availability of nutrients such as iron to limit microbial growth. However, Salmonella enterica serovar Typhimurium thrives in the inflamed gut by scavenging for iron with siderophores. By administering Escherichia coli strain Nissle 1917, which assimilates iron by similar mechanisms, we show that this nonpathogenic bacterium can outcompete and reduce S. Typhimurium colonization in mouse models of acute colitis and chronic persistent infection. This probiotic activity depends on E. coli Nissle iron acquisition, given that mutants deficient in iron uptake colonize the intestine but do not reduce S. Typhimurium colonization. Additionally, the ability of E. coli Nissle to overcome iron restriction by the host protein lipocalin 2, which counteracts some siderophores, is essential, given that S. Typhimurium is unaffected by E. coli Nissle in lipocalin 2-deficient mice. Thus, iron availability impacts S. Typhimurium growth, and E. coli Nissle reduces S. Typhimurium intestinal colonization by competing for this limiting nutrient. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|