The effect of levofloxacin concentration on the development and maintenance of antibiotic-resistant clones of Escherichia coli in chemostat culture
| Autor: | D. McCarthy, John W. Patching, Gerard T.A. Fleming, N Colombet |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Ofloxacin
medicine.drug_class Antibiotics Bioengineering Drug resistance Chemostat Levofloxacin Microbial Sensitivity Tests Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Antibiotic resistance Anti-Infective Agents Bacterial Proteins Drug Resistance Multiple Bacterial medicine Escherichia coli Selection Genetic Strain (chemistry) Culture Media bacteria Biotechnology medicine.drug |
| Zdroj: | Journal of industrial microbiologybiotechnology. 29(4) |
| ISSN: | 1367-5435 |
| Popis: | A levofloxacin-sensitive strain of Escherichia coli (broth MIC: 0.0625 mg x l(-1)) was grown in carbon-limited chemostat culture for 316 h (D=0.294 h(-1)). Hyperresistant strains isolated after 58 and 91 generations of culture retained a 16- to 47-fold increase in tolerance to levofloxacin during antibiotic-free serial batch and continuous culture (20 generations, glucose-limited, D=0.2 h(-1)). Isolates differed from the original strain in their maximum growth rates in the presence and absence of subinhibitory levels of levofloxacin, protein-banding profiles, and resistance to a range of antibiotics. Competition between resistant isolates and the original sensitive strain was studied in glucose-limited chemostat cultures (D=0.2 h(-1)). At levofloxacin concentrations less than 0.03 mg x l(-1), the sensitive strain outcompeted resistant isolates and displaced them from the culture, whereas the reverse was true at higher concentrations. These results have clinical and environmental implications for those administering levofloxacin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink