Validation of a phenol‐sulfuric acid method in a microplate format for the quantification of soluble sugars in ruminant feeds
| Autor: | Anuraga Jayanegara, Randi Mulianda, Masaya Matamura, Makoto Kondo, Naoto Isono, Toshiyuki Shibata, Takashi Mishima |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
03 medical and health sciences
chemistry.chemical_compound Ruminant Phenol Sugar alcohol Sugar 030304 developmental biology chemistry.chemical_classification 0303 health sciences Ethanol Chromatography biology Chemistry 0402 animal and dairy science Sulfuric acid 04 agricultural and veterinary sciences General Medicine Sulfuric Acids biology.organism_classification Animal Feed 040201 dairy & animal science Solubility Activated charcoal Charcoal Reagent Sugars General Agricultural and Biological Sciences Food Analysis |
| Zdroj: | Animal Science Journal. 92 |
| ISSN: | 1740-0929 1344-3941 |
| Popis: | Soluble sugars in feeds are important for ruminant production; however, performing numerous sugar analyses within a short period is a laborious task. Here, we developed a phenol-sulfuric acid (PSA) assay in a microplate format to quantify soluble sugars in ruminant feeds. This method is easy and quick and requires only a small quantity of harmful reagents. We found that assay measurements were not affected by the representative organic acids and sugar alcohol contained in feeds. The treatment of activated charcoal with ethanol extract prior to the PSA assay was effective in removing interfering compounds for a more accurate determination of soluble sugars in certain feeds. Furthermore, the inter-day and intra-day repeatability of the present method was acceptable. Hence, we conclude that the method developed in this study is suitable for routine analysis of soluble sugars content in ruminant feeds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text