Isolation, mapping, and characterization of trehalaseless mutants of Neurospora crassa
| Autor: | Malcolm Sargent, Alfred S. Sussman, M. Kerry Garrett, Shih-An Yu |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Genetics
Microbial Immunodiffusion Glycoside Hydrolases Mutant Acid Phosphatase Genetics and Molecular Biology Disaccharides Microbiology Neurospora Neurospora crassa chemistry.chemical_compound Glycoside hydrolase Trehalase Molecular Biology Crosses Genetic Recombination Genetic biology Genetic Complementation Test Chromosome Mapping biology.organism_classification Electrophoresis Disc Trehalose Molecular biology Culture Media Galactosidases Invertase Glucose Biochemistry chemistry Genes Amylases Mutation Carbohydrate Metabolism Maltase Glucosidases Sucrase |
| Zdroj: | Journal of bacteriology. 108(1) |
| ISSN: | 0021-9193 |
| Popis: | Mutant strains of Neurospora crassa that lack trehalase and are unable to grow on trehalose were isolated, and the gene ( tre ) was positioned on the right arm of linkage group I. Maltase and β-galactosidase activities are almost identical in tre − strains, whereas that of invertase was reduced by more than half and those of acid phosphatase and amylase were somewhat increased. Heterocaryons between standard and trehalaseless strains yield less than one-tenth the activity of the former. In addition, strains with duplications heterozygous for trehalase produce less than 1% of the activity of the standard strain. An inhibitor of trehalase has been found in tre − strains; its sensitivity to heat and proteolysis, and its nondialyzability suggest that this substance is a protein. The mig gene, which determines the rate of migration of trehalase on acrylamide gels, has been shown to be less than 1 map unit away from the tre gene. |
| Databáze: | OpenAIRE |
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