WIP/ITSN1 complex is involved in cellular vesicle trafficking and formation of filopodia-like protrusions
| Autor: | Alla Rynditch, Olga Gubar, Mariia Burdyniuk, S. V. Kropyvko, Tetyana Gryaznova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Endosome Wiskott-Aldrich Syndrome Protein Neuronal Transferrin receptor macromolecular substances Endosomes Biology Exocytosis 03 medical and health sciences Genetics Humans Pseudopodia Cytoskeleton Actin rab4 GTP-Binding Proteins Cellular Vesicle Cytoplasmic Vesicles Intracellular Signaling Peptides and Proteins Transferrin Biological Transport General Medicine Actins Cell biology Adaptor Proteins Vesicular Transport Cytoskeletal Proteins 030104 developmental biology HEK293 Cells Invadopodia MCF-7 Cells Filopodia |
| Zdroj: | Gene. 674 |
| ISSN: | 1879-0038 |
| Popis: | WIP (WASP interacting protein) together with N-WASP (neural Wiskott-Aldrich syndrome protein) regulates actin polymerization that is crucial for invadopodia and filopodia formation. Recently, we reported the WIP interaction with ITSN1 which is highly implicated in endo-/exocytosis, apoptosis, mitogenic signaling and cytoskeleton rearrangements. Here we demonstrate that the WIP/ITSN1 complex is involved in the transferrin receptor recycling and partially co-localizes with a marker of the fast recycling endosomes, RAB4. Moreover, ITSN1 recruits WIP to RAB4-positive vesicles upon overexpression. Our data indicate that WIP enhances the interaction of N-WASP with ITSN1 and promotes ITSN1/β-actin association. Moreover, the WIP/ITSN1-L complex facilitates formation of filopodia-like protrusions in MCF-7 cells. Thus, WIP/ITSN1 complex is involved in the cellular vesicle trafficking and actin-dependent membrane processes. |
| Databáze: | OpenAIRE |
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