The COP9 signalosome is essential for development of Drosophila melanogaster
| Autor: | Yael Nevo-Caspi, Shiri Freilich, Sara Orgad, Daniel A. Chamovitz, Daniel L. Segal, Efrat Oron, Ya'ara Kapp |
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| Rok vydání: | 1999 |
| Předmět: |
Saccharomyces cerevisiae
Arabidopsis Repressor Genes Insect General Biochemistry Genetics and Molecular Biology Species Specificity Two-Hybrid System Techniques Animals COP9 signalosome Protein Structure Quaternary Gene Plant Proteins Genetics Agricultural and Biological Sciences(all) biology COP9 Signalosome Complex Biochemistry Genetics and Molecular Biology(all) Proteins biology.organism_classification Drosophila melanogaster Proteasome Multiprotein Complexes Insect Proteins General Agricultural and Biological Sciences Function (biology) Peptide Hydrolases Signal Transduction |
| Zdroj: | Current Biology. 9:1187-S4 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/s0960-9822(00)80023-8 |
| Popis: | The COP9 signalosome (originally described as the COP9 complex) is an essential multi-subunit repressor of light-regulated development in plants [1] [2]. It has also been identified in mammals, though its role remains obscure [3] [4] [5]. This complex is similar to the regulatory lid of the proteasome and eIF3 [5] [9] [10] [11] [12] and several of its subunits are known to be involved in kinase signaling pathways [4] [6] [7] [8]. No proteins homologous to COP9 signalosome components were identified in the Saccharomyces cerevisiae genome, suggesting that the COP9 signalosome is specific for multi-cellular differentiation [13]. In order to reveal the developmental function of the COP9 signalosome in animals, we have isolated Drosophila melanogaster genes encoding eight subunits of the COP9 signalosome, and have shown by co-immunoprecipitation and gel-filtration analysis that these proteins are components of the Drosophila COP9 signalosome. Yeast two-hybrid assays indicated that several of these proteins interact, some through the PCI domain. Disruption of one of the subunits by either a P-element insertion or deletion of the gene caused lethality at the late larval or pupal stages. This lethality is probably a result of numerous pleiotropic effects. Our results indicate that the COP9 signalosome is conserved in invertebrates and that it has an essential role in animal development. |
| Databáze: | OpenAIRE |
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