Evidence for an interleukin-independent pathway for human lymphocyte activation
| Autor: | Ronald P. Daniele, Warner C. Greene, Peter C. Nowell, Gary A. Koretzky |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
DNA Replication
Interleukin 2 medicine.drug_class T-Lymphocytes Fluorescent Antibody Technique Endogeny Lymphocyte Activation Monoclonal antibody medicine Humans IL-2 receptor Calcimycin Cells Cultured Multidisciplinary biology Interleukin Lectin Molecular biology Anti-Bacterial Agents Mitogen-activated protein kinase biology.protein Interleukin-2 Antibody Research Article Interleukin-1 medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 80:3444-3447 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Though lectin mitogen stimulation of T-cell proliferation is an interleukin 1- (IL 1), interleukin 2- (IL 2) dependent process, the calcium ionophore A23187 may be able to initiate T-lymphocyte proliferation by an additional pathway. That the action of A23187 is IL 1 independent was demonstrated by its ability to stimulate monocyte-depleted cells without the addition of exogenous IL 1. The IL 2 independence of A23187 was indicated by (i) the inability of exogenous IL 2 to augment A23187-induced proliferation and (ii) the inability of the monoclonal antibody anti-Tac (with specificity for the human IL 2 receptor) to inhibit proliferation mediated by A23187. By contrast, in cultures stimulated with the lectin mitogen phytohemagglutinin, additional IL 2 considerably increased proliferation, whereas anti-Tac routinely caused 60-90% inhibition. Although the ionophore caused some IL 2 production and resulted in IL 2 receptor expression, quantitative studies showed that our results could not be explained by excessive amounts of endogenous IL 2 interfering with the blocking action of the antibody. Therefore, these data suggest that the action of A23187 as a human lymphocyte mitogen may be the result of at least two pathways--one dependent on the interleukin-cell interactions and the other independent of these mediators. |
| Databáze: | OpenAIRE |
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