Effects of 5-week ethanol feeding on the liver of aldehyde dehydrogenase 2 knockout mice
| Autor: | Tsunehiro Oyama, Masayoshi Ichiba, Akiko Matsumoto, Kyoko Kitagawa, Toshihiro Kawamoto, Fumihiro Mutoh, Toyohi Isse, Keiichi I. Nakayama |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Aldehyde dehydrogenase Acetaldehyde medicine.disease_cause Models Biological Mice chemistry.chemical_compound Internal medicine Genetics medicine Extracellular Animals General Pharmacology Toxicology and Pharmaceutics Molecular Biology Genetics (clinical) ALDH2 Mice Knockout Ethanol biology Tumor Necrosis Factor-alpha Aldehyde Dehydrogenase Mitochondrial Alanine Transaminase Aldehyde Dehydrogenase Malondialdehyde Animal Feed Mice Inbred C57BL Oxidative Stress Endocrinology Liver chemistry Biochemistry Alanine transaminase biology.protein Molecular Medicine Female Oxidative stress |
| Zdroj: | Pharmacogenetics and Genomics. 18:847-852 |
| ISSN: | 1744-6872 |
| DOI: | 10.1097/fpc.0b013e328307a0a9 |
| Popis: | Objective The polymorphism of aldehyde dehydrogenase 2 (ALDH2), denoted ALDH2*2, is very common in East Asian countries, and the mutated ALDH2 protein derived from ALDH2*2 lacks the ability of acetaldehyde metabolization. Our aim was to determine the consequences of the ALDH2 polymorphism on ethanol-administered liver tissue. Methods Aldh2+/+, +/−, and −/− mice were fed with ethanol solution and standard hard feed for 5 weeks. Results The serum alanine aminotransferase (ALT) level in the Aldh2−/− mice clearly decreased upon ethanol feeding, in contrast to Aldh2+/+ mice, in which the ALT level was unchanged. The levels of malondialdehyde, phospho extracellular signal-regulated kinase 2, and tumor necrosis factor-alpha in the liver tissue all correlated with the ALT level. Conclusion These findings suggest that ethanol intake in the presence of inactive ALDH2 decreases the serum ALT level following a decrease in oxidative stress and tumor necrosis factor-alpha secretion. |
| Databáze: | OpenAIRE |
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