Mutants of T7 bacteriophage inhibited by lambda prophage
| Autor: | J. F. Speyer, Chia C. Pao |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
DNA Replication
chemistry.chemical_classification Genetics DNA ligase Mutation Multidisciplinary viruses Mutant Chromosome Mapping biochemical phenomena metabolism and nutrition Biology Virus Replication medicine.disease_cause Coliphages Molecular biology Complementation Lysogen chemistry Lysogenic cycle medicine Lysogeny Gene Prophage Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 72:3642-3646 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.72.9.3642 |
| Popis: | Mutants in gene 20, a new T7 gene, cannot grow on rex+ lambda lysogens. Gene 20-- mutants suppress in double mutants the phenotype of T7 ligase negative mutations, but not vice versa. Amber 20- mutants have been obtained. There are differences between these T7 mutations and the similar T4 rII mutations. There are host mutations which permit T7 20- mutants to grow on lambda+ lysogens. T7 DNA synthesis on normal lambda+ lysogens infected with 20- mutants is essentially normal, but the DNA is not packaged. The gene 20 protein is active in in vitro complementation and probably used late in infection for DNA packaging into phage heads. |
| Databáze: | OpenAIRE |
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