A monoclonal antibody that detects a polymorphic determinant common to HLA-DR1 and 2
| Autor: | Miki Aizawa, Kazumasa Ogasawara, Hitoshi Ikeda, Yuko Kikuchi, Masanori Kasahara, Akemi Wakisaka, T. Okuyama, T Takenouchi, N. Ishikawa |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.drug_class
HLA-DR1 Genes MHC Class II Immunology chemical and pharmacologic phenomena Biology Monoclonal antibody Biochemistry Subclass Cell Line Epitopes Mice Genetics medicine Animals Humans Immunology and Allergy Cytotoxic T cell B cell HLA Complex B-Lymphocytes Mice Inbred BALB C Polymorphism Genetic medicine.diagnostic_test HLA-DR1 Antigen Histocompatibility Antigens Class II Antibodies Monoclonal HLA-DR Antigens General Medicine Molecular biology Complement-dependent cytotoxicity medicine.anatomical_structure Female Binding Sites Antibody Tissue typing |
| Zdroj: | Tissue Antigens. 21:105-113 |
| ISSN: | 1399-0039 0001-2815 |
| DOI: | 10.1111/j.1399-0039.1983.tb00378.x |
| Popis: | In an attempt to study the gene products of the HLA complex, a monoclonal antibody, named HU-30, was produced by immunizing BALB/c mice with a cultured human B lymphoblastoid cell line, Shi-C3 (Aw24, Aw31, Bw51, Bw52, DR2, DR blank, MT1, MT2, MB3). HU-30 belonged to the IgG2 subclass and was active in complement dependent cytotoxicity. When the serological specificity was evaluated with a panel of 15 cultured human lymphoblastoid cell lines, it was found that HU-30 detected a polymorphic determinant, common to HLA-DR1 and 2, with much stronger cytotoxic activity against HLA-DR2 positive B cell lines. When HU-30 was tested against a panel of B cells from 84 healthy donors at a dilution of 2(-13), it gave positive reactions only with cells typed as HLA-DR2. Furthermore, sequential coprecipitation studies indicated that the HU-30 determinant was borne on the molecules carrying the HLA-DR determinants. Thus, HU-30 appears to be of great value as a tissue typing reagent monospecific for HLA-DR2. |
| Databáze: | OpenAIRE |
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