Characterization of extrathymic CD8alphabeta T cells in the liver and intestine in TAP-1 deficient mice
| Autor: | Toru Abo, Hiroki Kawamura, Chikako Miyaji, Hisashi Yokoyama, Yuiko Ishimoto, Ryoko Miyakawa, Shinobu Miyazawa, Hisami Watanabe, Chika Tsukada |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cytotoxicity
Immunologic Aging Isoantigens CD3 Complex Receptors Antigen T-Cell alpha-beta T cell Lymphocyte Immunology CD1 Spleen CD8-Positive T-Lymphocytes Biology Mice Antigen T-Lymphocyte Subsets Intestine Small medicine Animals Immunology and Allergy Cytotoxic T cell ATP Binding Cassette Transporter Subfamily B Member 2 Pan-T antigens Cells Cultured Mice Inbred BALB C Histocompatibility Antigens Class I Original Articles Molecular biology Mice Inbred C57BL medicine.anatomical_structure Liver ATP-Binding Cassette Transporters Lymphocyte Culture Test Mixed Cell Division CD8 |
| Zdroj: | Immunology. 109:343-350 |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1046/j.1365-2567.2003.01654.x |
| Popis: | TAP-1 deficient (-/-) mice cannot transport MHC class I antigens onto the cell surface, which results in failure of the generation of CD8+ T cells in the thymus. In a series of recent studies, it has been proposed that extrathymic T cells are generated in the liver and at other extrathymic sites (e.g. the intestine). It was therefore investigated whether CD8+ extrathymic T cells require an interaction with MHC class I antigens for their differentiation in TAP-1(-/-) mice. Although CD8+ thymically derived T cells were confirmed to be absent in the spleen as well as in the thymus, CD8 alpha beta+ T cells were abundant in the livers and intestines of TAP-1(-/-) mice. These CD8+ T cells expanded in the liver as a function of age and were mainly confined to a NK1.1-CD3int population which is known to be truly of extrathymic origin. Hepatic lymphocytes, which contained CD8+ T cells and which were isolated from TAP-1(-/-) mice (H-2b), responded to neither mutated MHC class I antigens (bm1) nor allogeneic MHC class I antigens (H-2d) in in vitro mixed lymphocyte cultures. However, the results from repeated in vivo stimulations with alloantigens (H-2d) were interesting. Allogeneic cytotoxicity was induced in liver lymphocytes in TAP-1(-/-) mice, although the magnitude of cytotoxicity was lower than that of liver lymphocytes in immunized B6 mice. All allogeneic cytotoxicity disappeared with the elimination of CD8+ cells in TAP-1(-/-) mice. These results suggest that the generation and function of CD8+ extrathymic T cells are independent of the existence of the MHC class I antigens of the mouse but have a limited allorecognition ability. |
| Databáze: | OpenAIRE |
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