Nerve Growth Factor gene ovarian expression, polymorphism identification, and association with litter size in goats
| Autor: | K.C. Raghavan, T.V. Aravindakshan, K. Shyama, R. T. Venkatachalapathy, M. Mini, Thomas Naicy, G. Radhika |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Litter (animal) Genetic Markers Litter Size Population Single-nucleotide polymorphism Biology 03 medical and health sciences Exon Food Animals Polymorphism (computer science) Gene expression Nerve Growth Factor Animals Small Animals education Gene Genetics education.field_of_study Polymorphism Genetic Base Sequence Equine Goats Ovary Single-strand conformation polymorphism 030104 developmental biology Gene Expression Regulation Animal Science and Zoology Female |
| Zdroj: | Theriogenology. 86(9) |
| ISSN: | 1879-3231 |
| Popis: | The Nerve Growth Factor (NGF) plays an important role in reproduction by augmenting folliculogenesis. In this study, the coding regions of caprine NGF gene were analyzed to detect single-nucleotide polymorphisms (SNPs), their association with litter size, and the relative ovarian expression of NGF gene in the two indigenous goat breeds of South India viz., the prolific Malabari and less-prolific Attappady Black. The sequence analysis of the third exon containing the entire open reading frame of NGF gene was observed to be of 808 bp with one nonsynonymous mutation at 217th position. Later, polymerase chain reaction (PCR) was performed to amplify a region of 188 bp covering the region carrying the detected mutation. The genomic DNAs from the goats under study (n = 277) were subjected to PCR and single strand conformation polymorphism (SSCP). On analysis, four diplotypes viz., AA, AB, AC, and AD were observed with respective frequencies of 0.50, 0.22, 0.27, and 0.01. Sequencing of the representative samples revealed an additional synonymous mutation, i.e., g.291C>A. Statistical analysis indicated that NGF diplotypes and the SNP g.217G>A were associated with litter size in goats (P |
| Databáze: | OpenAIRE |
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