A plasmid encoding a combination of mosquito-larvicidal genes fromBacillus thuringiensissubsp.israelensisandBacillus sphaericusconfers toxicity against a broad range of mosquito larvae when expressed in Gram-negative bacteria
| Autor: | Plearnpis Luxananil, Sakol Panyim, Mongkon Audtho, Boonhiang Promdonkoy, Sutipa Tanapongpipat, Namchai Chewawiwat |
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| Rok vydání: | 2003 |
| Předmět: |
Insecticides
Operon Bacterial Toxins Bacillus thuringiensis Gene Expression Bacillus medicine.disease_cause Microbiology Bacillus sphaericus Hemolysin Proteins Plasmid Bacterial Proteins Aedes Anopheles Escherichia coli Genetics medicine Animals Cloning Molecular Pest Control Biological Promoter Regions Genetic Molecular Biology Bacillaceae Bacillus thuringiensis Toxins biology fungi biology.organism_classification Bacillales Recombinant Proteins Endotoxins Culex Transformation (genetics) Culicidae Genes Bacterial Larva Transformation Bacterial Plasmids |
| Zdroj: | FEMS Microbiology Letters. 228:259-263 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1016/s0378-1097(03)00780-8 |
| Popis: | A recombinant plasmid harboring cry4A, cry4B and cry11A from Bacillus thuringiensis subsp. israelensis and binary toxin genes from Bacillus sphaericus has been constructed. The three cry genes were placed under the control of the cry4B promoter whereas the binary toxin gene was controlled by its native promoter. The expression of toxins in Escherichia coli harboring the resulting plasmid, p4BDA-5142, was investigated. Cry4B expression was highest compared to other toxins. Although the level of toxin expression was low compared with E. coli expressing single toxins, the recombinant E. coli strain harboring p4BDA-5142 exhibited broad range mosquito-larvicidal activity against all Aedes, Culex and Anopheles larvae. This work has shown that the development of the recombinant plasmid can be used to broaden the host range spectrum of the appropriate bacterial host for mosquito control. |
| Databáze: | OpenAIRE |
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