Chondroitin sulfate reduces the friction coefficient of articular cartilage
| Autor: | Faye H. Chen, Gerard A. Ateshian, Michael Kaplun, Nadeen O. Chahine, Ines M. Basalo, Clark T. Hung |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
musculoskeletal diseases
Cartilage Articular Friction Biomedical Engineering Biophysics Mineralogy Polyethylene glycol Osteoarthritis In Vitro Techniques Models Biological Article chemistry.chemical_compound Viscosity PEG ratio medicine Osmotic pressure Animals Orthopedics and Sports Medicine Computer Simulation Chondroitin sulfate Chromatography Osmotic concentration Dose-Response Relationship Drug Cartilage Rehabilitation Chondroitin Sulfates musculoskeletal system medicine.disease medicine.anatomical_structure chemistry Cattle |
| Zdroj: | Journal of biomechanics. 40(8) |
| ISSN: | 0021-9290 |
| Popis: | Summary The objective of this study was to investigate the effect of chondroitin sulfate (CS)-C on the frictional response of bovine articular cartilage. The main hypothesis is that CS decreases the friction coefficient of articular cartilage. Corollary hypotheses are that viscosity and osmotic pressure are not the mechanisms that mediate the reduction in the friction coefficient by CS. In Experiment 1, bovine articular cartilage samples ( n =29) were tested in either phosphate buffered saline (PBS) or in PBS containing 100 mg/ml of CS following 48 h incubation in PBS or in PBS+100 mg/ml CS (control specimens were not subjected to any incubation). In Experiment 2, samples ( n =23) were tested in four different solutions: PBS, PBS+100 mg/ml CS, and PBS+polyethylene glycol (PEG) (133 or 170 mg/ml). In Experiment 3, samples ( n =18) were tested in three solutions of CS (0, 10 and 100 mg/ml). Frictional tests (cartilage-on-glass) were performed under constant stress (0.5 MPa) for 3600 s and the time-dependent friction coefficient was measured. Samples incubated or tested in a 100 mg/ml CS solution exhibited a significantly lower equilibrium friction coefficient than the respective PBS control. PEG solutions delayed the rise in the friction coefficient relative to the PBS control, but did not reduce the equilibrium value. Testing in PBS+10 mg/ml of CS did not cause any significant decrease in the friction coefficient. In conclusion, CS at a concentration of 100 mg/ml significantly reduces the friction coefficient of bovine articular cartilage and this mechanism is neither mediated by viscosity nor osmolarity. These results suggest that direct injection of CS into the joint may provide beneficial tribological effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect