A Glial Cell Line-Derived Neurotrophic Factor-Secreting Clone of the Schwann Cell Line SCTM41 Enhances Survival and Fiber Outgrowth from Embryonic Nigral Neurons Grafted to the Striatum and to the Lesioned Substantia Nigra
| Autor: | Kathryn H. Adcock, John H. Rogers, Elizabeth M. Muir, Simon R. Sinclair, James W. Fawcett, Martin J. Wilby, Stephen B. Dunnett, Rike Zietlow, Philippe Horellou |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Neurite
Dopamine Schwann cell Substantia nigra Nerve Tissue Proteins Transfection Article Cell Line Nerve Fibers Neurotrophic factors Mesencephalon Dopaminergic Cell Glial cell line-derived neurotrophic factor medicine Animals Glial Cell Line-Derived Neurotrophic Factor Nerve Growth Factors Neurons biology General Neuroscience Dopaminergic Graft Survival Coculture Techniques Corpus Striatum Clone Cells Rats Substantia Nigra medicine.anatomical_structure Nerve growth factor nervous system biology.protein Schwann Cells Neuroscience Biomarkers |
| Popis: | We have developed a novel Schwann cell line, SCTM41, derived from postnatal sciatic nerve cultures and have stably transfected a clone with a rat glial cell line-derived neurotrophic factor (GDNF) construct. Coculture with this GDNF-secreting clone enhancesin vitrosurvival and fiber growth of embryonic dopaminergic neurons. In the rat unilateral 6-OHDA lesion model of Parkinson’s disease, we have therefore made cografts of these cells with embryonic day 14 ventral mesencephalic grafts and assayed for effects on dopaminergic cell survival and process outgrowth. We show that cografts of GDNF-secreting Schwann cell lines improve the survival of intrastriatal embryonic dopaminergic neuronal grafts and improve neurite outgrowth into the host neuropil but have no additional effect on amphetamine-induced rotation. We next looked to see whether bridge grafts of GDNF-secreting SCTM41 cells would promote the growth of axons to their striatal targets from dopaminergic neurons implanted orthotopically into the 6-OHDA-lesioned substantia nigra. We show that such bridge grafts increase the survival of implanted embryonic dopaminergic neurons and promote the growth of axons through the grafts to the striatum. |
| Databáze: | OpenAIRE |
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