IN VITRO PASSIVE SENSITIZATION OF THE URETER AS A BASIS FOR THE STUDY OF NONINFECTIOUS URETERAL INFLAMMATION
| Autor: | Stephen Y. Nakada, David T. Uehling, Ricardo Saban, Matthew J. Zine, Dale E. Bjorling |
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| Rok vydání: | 1998 |
| Předmět: |
Leukotriene
medicine.medical_specialty Contraction (grammar) business.industry medicine.medical_treatment Urology Inflammation Nephrectomy Pathophysiology chemistry.chemical_compound Ureter medicine.anatomical_structure Endocrinology chemistry Internal medicine medicine medicine.symptom business Histamine Sensitization |
| Zdroj: | The Journal of Urology. :1924-1927 |
| ISSN: | 0022-5347 |
| DOI: | 10.1097/00005392-199811000-00085 |
| Popis: | Purpose: To develop an in vitro model of passive sensitization for the ureter for the study of noninfectious ureteral inflammation.Materials and Methods: Human ureteral tissues were obtained from excess segments of ureters from patients undergoing donor nephrectomy. Following excision, ureters were placed in physiologic salt solution (PSS) and passively sensitized by incubating with ragweed serum from allergic donor (1 ml. serum: 4 ml. PSS) for 20 hours at room temperature. Ureteral segments were incubated with PSS only and served as non-sensitized controls (n = 4). After sensitization, excess serum was removed by serial washing with PSS without serum. Ureteral strips were then suspended in vitro for determination of tissue contraction. Contractile responses and histamine release were measured. Tissues were then exposed to antigen.To investigate the role of inflammatory mediators in tissue contraction, 4 groups of 8 sensitized ureteral segments were incubated for 1 hour with the following substanc... |
| Databáze: | OpenAIRE |
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