A Gene-alteration Profile of Human Lung Cancer Cell Lines
| Autor: | Ruben Pio, Raquel Blanco, Moying Tang, Barbara Angulo, Takashi Kohno, Jun Yokota, Montse Sanchez-Cespedes, Reika Iwakawa, John D. Minna, Luis M. Montuenga |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Neuroblastoma RAS viral oncogene homolog Oncogens Lung Neoplasms Blotting Western Tumor suppressors Biology medicine.disease_cause Polymerase Chain Reaction Article Cell Line Tumor Genetics medicine Humans PTEN Epidermal growth factor receptor Lung cancer neoplasms Genetics (clinical) PI3K/AKT/mTOR pathway Tyrosine kinase inhibitors Gene Expression Profiling Cancer Oncogenes Middle Aged medicine.disease Mutation biology.protein Cancer research Càncer de pulmó Female Erlotinib KRAS medicine.drug |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Recercat. Dipósit de la Recerca de Catalunya instname Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Popis: | Aberrant proteins encoded from genes altered in tumors drive cancer development and may also be therapeutic targets. Here we derived a comprehensive gene-alteration profile of lung cancer cell lines. We tested 17 genes in a panel of 88 lung cancer cell lines and found the rates of alteration to be higher than previously thought. Nearly all cells feature inactivation at TP53 and CDKN2A or RB1, whereas BRAF, MET, ERBB2, and NRAS alterations were infrequent. A preferential accumulation of alterations among histopathological types and a mutually exclusive occurrence of alterations of CDKN2A and RB1 as well as of KRAS, epidermal growth factor receptor (EGFR), NRAS, and ERBB2 were seen. Moreover, in nonsmall- cell lung cancer (NSCLC), concomitant activation of signal transduction pathways known to converge in mammalian target of rapamycin (mTOR) was common. Cells with single activation of ERBB2, PTEN, or MET signaling showed greater sensitivity to cell-growth inhibition induced by erlotinib, LY294002, and PHA665752, respectively, than did cells featuring simultaneous activation of these pathways, underlining the need for combined therapeutic strategies in targeted cancer treatments. In conclusion, our gene-alteration landscape of lung cancer cell lines provides insights into how gene alterations accumulate and biological pathways interact in cancer. Hum Mutat 30, 1199–1206, 2009. & 2009Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|