Functional expression of IL-12 receptor by human eosinophils: IL-12 promotes eosinophil apoptosis
| Autor: | Abdelillah Soussi-Gounni, Fadi Aris, Qutayba Hamid, Esra Nutku, Bruce Mazer, Qianli Zhuang |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Immunology
Apoptosis Cell Separation Allergic inflammation chemistry.chemical_compound medicine Immunology and Allergy Eosinophilia Humans Propidium iodide Viability assay RNA Messenger Cells Cultured Eosinophil cationic protein biology Cell Membrane Receptors Interleukin-12 Receptors Interleukin Eosinophil Immunohistochemistry Interleukin-12 Cell biology Eosinophils medicine.anatomical_structure chemistry Major basic protein biology.protein Tetradecanoylphorbol Acetate medicine.symptom Interleukin-5 |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0022-1767 |
| Popis: | In murine models of allergic inflammation, IL-12 has been shown to decrease tissue eosinophilia, but the underlying mechanisms are not known. We evaluated the expression of IL-12R and the effect of IL-12 on eosinophil survival. In situ hybridization demonstrated the presence of mRNA and immunoreactivity for IL-12Rβ1 and -β2 subunits in human peripheral blood eosinophils. Surface expression of IL-12Rβ1 and -β2 subunits on freshly isolated human eosinophils was optimally expressed after incubation with PMA. To determine the functional significance of IL-12R studies, we studied cell viability and apoptosis. Morphological analysis and propidium iodide staining for cell cycle demonstrated that recombinant human IL-12 increased in vitro human eosinophil apoptosis in a dose-dependent manner. Addition of IL-5 together with IL-12 abrogated eosinophil apoptosis, suggesting that IL-12 and IL-5 have antagonistic effects. Our findings provide evidence for a novel role for IL-12 in regulating eosinophil function by increasing eosinophil apoptosis. |
| Databáze: | OpenAIRE |
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