Syringyl lignin production in conifers: Proof of concept in a Pine tracheary element system
| Autor: | Barbara Geddes, Fachuang Lu, Armin Wagner, John Ralph, Lorelle Phillips, Heather Flint, Yuki Tobimatsu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Magnetic Resonance Spectroscopy
Softwood Materials science Polymers Radiata Lignin Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Cotransformation Cell Wall Gene Expression Regulation Plant Botany Caffeic acid Hardwood Biomass Transgenes Plant Proteins Multidisciplinary biology Pinus radiata Biological Sciences Pinus Plants Genetically Modified biology.organism_classification Tracheophyta Metabolic Engineering chemistry Sinapyl alcohol Alcohols Biofuels |
| Zdroj: | Proceedings of the National Academy of Sciences. 112:6218-6223 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Conifers (softwoods) naturally lack syringyl units in their lignins, rendering lignocellulosic materials from such species more difficult to process than syringyl-rich hardwood species. Using a transformable Pinus radiata tracheary element (TE) system as an experimental platform, we investigated whether metabolic engineering can be used to create syringyl lignin in conifers. Pyrolysis-GC/MS and 2D-NMR analysis of P. radiata TE cultures transformed to express ferulate 5-hydroxylase (F5H) and caffeic acid O-methyltransferase (COMT) from Liquidambar styraciflua confirmed the production and incorporation of sinapyl alcohol into the lignin polymer. Transformation with F5H was sufficient for the production of syringyl lignin in TEs, but cotransformation with COMT improved its formation. In addition, lower levels of the pathway intermediate 5-hydroxyconiferyl alcohol were evidenced in cotransformation experiments, indicating that the introduction of the COMT overcame the inefficiency of the native pine methyltransferases for supporting sinapyl alcohol production.Our results provide the proof of concept that it is possible to generate a lignin polymer that contains syringyl units in softwood species such as P. radiata, suggesting that it might be possible to retain the outstanding fiber properties of softwoods while imbuing them with the lignin characteristics of hardwoods that are more favorable for industrial processing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|