TiO 2 -NPs and cadmium co-exposure: in vitro assessment of genetic and genomic DNA damage on Dicentrarchus labrax embryonic cells
| Autor: | Giada Frenzilli, Marianna Santonastaso, V. Genualdo, Simona Picchietti, Concetta Iovine, Lucia Rocco, Filomena Mottola |
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| Přispěvatelé: | Mottola, F., Santonastaso, M., Iovine, C., Frenzilli, G., Picchietti, S., Genualdo, V., Rocco, L. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
DNA damage
Health Toxicology and Mutagenesis Environmental pollution medicine.disease_cause chemistry.chemical_compound medicine Environmental Chemistry biology Chemistry Contaminant absorption technology industry and agriculture Titanium dioxide nanoparticles Dicentrarchus labrax embryonic cells (DLEC) General Medicine biology.organism_classification Pollution Molecular biology In vitro Comet assay Apoptosis Dicentrarchus Genotoxicity DNA Cadmium |
| Zdroj: | Environmental science and pollution research international (2021). doi:10.1007/s11356-021-17645-6 info:cnr-pdr/source/autori:Filomena Mottola, Marianna Santonastaso, Concetta Iovine, Giada Frenzilli, Simona Picchietti, Viviana Genualdo and Lucia Rocco/titolo:TiO 2-NPs and cadmium co-exposure: in vitro assessment of genetic and genomic DNA damage on Dicentrarchus labrax embryonic cells/doi:10.1007%2Fs11356-021-17645-6/rivista:Environmental science and pollution research international/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| Popis: | The increased titanium dioxide nanoparticles (TiO2-NPs) spread and their interaction with organic and inorganic pollutants arouses concern for the potential hazards for organisms and environment. This study tested in vitro the genotoxic effects of TiO2-NPs (1 μg/mL) and cadmium (Cd) (0.1 μg/mL) co-exposure using Dicentrarchus labrax embryonic cells (DLEC) as experimental model. The genotoxicity tests (Comet assay, Diffusion Assay and Random Amplification of Polymorphic DNA (RAPD-PCR) were conducted after 3, 24 and 48 hours of exposure to TiO2-NPs and Cd alone and in combination. The results showed that the percentage of DNA damage and apoptotic cells increases following 48 hours TiO2-NPs exposure, while DNA instability was detected for all the times tested. Cd induced genotoxic effects starting from 3 hour-exposure and for all the treatment times. Cd + TiO2-NPs co-exposure did not cause any genomic damage or apoptosis for all the exposure times. The possibility that Cd and TiO2-NPs form aggregates no longer able of penetrating the nucleus and damaging the genetic material is discussed. Graphical abstract: [Figure not available: see fulltext.]. |
| Databáze: | OpenAIRE |
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