Molecular analysis of antigen receptor variable region repertoires in T lymphocytes infiltrating the intrathyroidal and extrathyroidal manifestations in patients with Graves' disease*
| Autor: | Armin E. Heufelder, Rebecca S. Bahn, James A. Garrity, B. E. Wenzel, H.-D. Schworm |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Eye Diseases
T-Lymphocytes Endocrinology Diabetes and Metabolism T cell Graves' disease Receptors Antigen T-Cell Thyroid Gland Biology Gene Rearrangement T-Lymphocyte Skin Diseases Endocrinology Antigen Internal Medicine medicine Humans Tibia Pretibial myxedema T-cell receptor Thyroid General Medicine T lymphocyte Gene rearrangement medicine.disease Graves Disease medicine.anatomical_structure Immunology Orbit |
| Zdroj: | Experimental and Clinical Endocrinology & Diabetes. 104:84-87 |
| ISSN: | 1439-3646 0947-7349 |
| Popis: | To determine whether T cells infiltrating thyroid, orbital and pretibial tissue of patients with Graves' ophthalmopathy (GO) and pretibial dermopathy (PTD) represent a primary immune response that is directed against certain antigenic determinants shared between these involved tissues, we characterized these T cells at the molecular level. T cell antigen receptor (TcR) variable (V) region gene usage in thyroid, orbital, pretibial tissue and peripheral blood mononuclear cells of patients with GD, GO and PTD was assessed using RT-PCR and 22 V alpha and 23 V beta gene-specific oligonucleotide primers, followed by Southern hybridization analysis using TcR C-region-specific, digoxigenin-labelled oligonucleotide probes. In some instances, CDR3- and junctional regions of TcR V beta genes were sequenced. Marked restriction and similarities of V alpha and V beta gene usage were detected in samples derived from patients with active GO and PTD of recent onset. Moreover, sequence analysis of junctional domains of V beta families revealed oligoclonality of some intrathyroidal, orbital and pretibial T cell populations as well as the presence of conserved junctional motifs shared by T cells derived the thyroid gland and the extrathyroidal sites. These data suggest that similar antigenic determinants may be responsible for the recruitment and oligoclonal expansion of T cells both within the thyroid gland and at the involved extrathyroidal sites in Graves' disease. |
| Databáze: | OpenAIRE |
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