Detection of Latin American Strains of Histoplasma in a Murine Model by Use of a Commercially Available Antigen Test
Autor: | McCall S, Duane R. Hospenthal, Flanagan Jp, L.J. Wheat, Allton Dr, Rivard Rg, Boyd Tm, Michelle Durkin, Patricia Connolly |
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Rok vydání: | 2010 |
Předmět: |
Microbiology (medical)
Antigens Fungal Histoplasma Clinical Biochemistry Immunology chemical and pharmacologic phenomena complex mixtures Sensitivity and Specificity Histoplasmosis Mice fluids and secretions Antigen Chiroptera parasitic diseases medicine Animals Clinical Laboratory Immunology Immunology and Allergy Clade Immunoassay Mice Inbred ICR biology medicine.diagnostic_test Clinical Laboratory Techniques Outbreak bacterial infections and mycoses medicine.disease biology.organism_classification Virology United States Murine model Animals Zoo Female Restriction fragment length polymorphism |
Zdroj: | Clinical and Vaccine Immunology. 17:802-806 |
ISSN: | 1556-679X 1556-6811 |
Popis: | During a Histoplasma outbreak in a colony of fruit bats at a southern United States zoo, it was observed that although Histoplasma was recovered in culture from multiple sites at necropsy, none of the samples collected from those bats tested positive for Histoplasma antigen (HAg). Five of the Histoplasma isolates from the bats were subsequently identified as Latin American (LA) clade A, restriction fragment length polymorphism (RFLP) class 6. These observations raised concern as to whether the commercially available HAg test could detect Histoplasma antigen not of the North American clade upon which the HAg test had been developed. To evaluate this concern, a murine model of disseminated histoplasmosis was established, and mice were infected with multiple LA Histoplasma isolates, including clinical isolates recovered from Brazilian AIDS patients (RFLP class 5 and class 6) and isolates recovered from the bats during the outbreak (RFLP class 6). Histoplasma antigen was detected in all infected mice in our experiments, even when Histoplasma was not recovered in culture. Because the currently available HAg test is able to detect Histoplasma antigen in mice infected with Latin American isolates, this suggests that bat host factors rather than differences among Histoplasma RFLP classes were responsible for the inability to detect HAg in infected bats. |
Databáze: | OpenAIRE |
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