Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats
Autor: | Yu Wen Cheng, George Hsiao, Wei Jan Huang, Cheng Hui Lin, Man Ru Wu, Diana Shu Lian Chow |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Retinal degeneration Light genetic structures Cell Culture Techniques Apoptosis Retinal Pigment Epithelium lcsh:Chemistry N-retinylidene-N-retinylethanolamine (A2E) chemistry.chemical_compound 0302 clinical medicine lcsh:QH301-705.5 Spectroscopy Neovascularization Pathologic Retinal Degeneration General Medicine Computer Science Applications medicine.anatomical_structure lipids (amino acids peptides and proteins) Phototoxicity retinal damage Catalysis Article Lipofuscin Cell Line Inorganic Chemistry 03 medical and health sciences medicine Animals Physical and Theoretical Chemistry Molecular Biology Retina Tight Junction Proteins Organic Chemistry Retinal medicine.disease Molecular biology blue light eye diseases Rats 030104 developmental biology chemistry lcsh:Biology (General) lcsh:QD1-999 Cell culture 030221 ophthalmology & optometry retinal pigment epithelial cells (RPE) Neuron sense organs |
Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 7 International Journal of Molecular Sciences, Vol 20, Iss 7, p 1799 (2019) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms20071799 |
Popis: | N-retinylidene-N-retinylethanolamine (A2E) and other bisretinoids are components of lipofuscin and accumulate in retinal pigment epithelial (RPE) cells&mdash these adducts are recognized in the pathogenesis of retinal degeneration. Further, blue light-emitting diode (LED) light (BLL)-induced retinal toxicity plays an important role in retinal degeneration. Here, we demonstrate that low-luminance BLL enhances phototoxicity in A2E-laden RPE cells and rats. RPE cells were subjected to synthetic A2E, and the effects of BLL on activation of apoptotic biomarkers were examined by measuring the levels of cleaved caspase-3. BLL modulates the protein expression of zonula-occludens 1 (ZO-1) and paracellular permeability in A2E-laden RPE cells. Early inflammatory and angiogenic genes were also screened after short-term BLL exposure. In this study, we developed a rat model for A2E treatment with or without BLL exposure for 21 days. BLL exposure caused fundus damage, decreased total retinal thickness, and caused neuron transduction injury in the retina, which were consistent with the in vitro data. We suggest that the synergistic effects of BLL and A2E accumulation in the retina increase the risk of retinal degeneration. These outcomes help elucidate the associations between BLL/A2E and angiogenic/apoptotic mechanisms, as well as furthering therapeutic strategies. |
Databáze: | OpenAIRE |
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