Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats

Autor: Yu Wen Cheng, George Hsiao, Wei Jan Huang, Cheng Hui Lin, Man Ru Wu, Diana Shu Lian Chow
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Retinal degeneration
Light
genetic structures
Cell Culture Techniques
Apoptosis
Retinal Pigment Epithelium
lcsh:Chemistry
N-retinylidene-N-retinylethanolamine (A2E)
chemistry.chemical_compound
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
Neovascularization
Pathologic
Retinal Degeneration
General Medicine
Computer Science Applications
medicine.anatomical_structure
lipids (amino acids
peptides
and proteins)
Phototoxicity
retinal damage
Catalysis
Article
Lipofuscin
Cell Line
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
Retina
Tight Junction Proteins
Organic Chemistry
Retinal
medicine.disease
Molecular biology
blue light
eye diseases
Rats
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Cell culture
030221 ophthalmology & optometry
retinal pigment epithelial cells (RPE)
Neuron
sense organs
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 7
International Journal of Molecular Sciences, Vol 20, Iss 7, p 1799 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20071799
Popis: N-retinylidene-N-retinylethanolamine (A2E) and other bisretinoids are components of lipofuscin and accumulate in retinal pigment epithelial (RPE) cells&mdash
these adducts are recognized in the pathogenesis of retinal degeneration. Further, blue light-emitting diode (LED) light (BLL)-induced retinal toxicity plays an important role in retinal degeneration. Here, we demonstrate that low-luminance BLL enhances phototoxicity in A2E-laden RPE cells and rats. RPE cells were subjected to synthetic A2E, and the effects of BLL on activation of apoptotic biomarkers were examined by measuring the levels of cleaved caspase-3. BLL modulates the protein expression of zonula-occludens 1 (ZO-1) and paracellular permeability in A2E-laden RPE cells. Early inflammatory and angiogenic genes were also screened after short-term BLL exposure. In this study, we developed a rat model for A2E treatment with or without BLL exposure for 21 days. BLL exposure caused fundus damage, decreased total retinal thickness, and caused neuron transduction injury in the retina, which were consistent with the in vitro data. We suggest that the synergistic effects of BLL and A2E accumulation in the retina increase the risk of retinal degeneration. These outcomes help elucidate the associations between BLL/A2E and angiogenic/apoptotic mechanisms, as well as furthering therapeutic strategies.
Databáze: OpenAIRE
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