Growth and differentiation in vitro of the accumulating Lyt-2-/L3T4- subset in lpr mice
| Autor: | R C, Budd, H R, MacDonald, J W, Lowenthal, J L, Davignon, S, Izui, J C, Cerottini |
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| Jazyk: | angličtina |
| Rok vydání: | 1985 |
| Předmět: |
Immunology
Receptors Immunologic/metabolism chemical and pharmacologic phenomena Cell Cycle/drug effects ddc:616.07 Lymphocyte Activation Mice immune system diseases Concanavalin A/pharmacology Concanavalin A Antigens Ly/genetics Antigens Ly Immunology and Allergy Animals Antigens Surface/genetics Receptors Immunologic Cell Cycle Tetradecanoylphorbol Acetate/pharmacology Cell Differentiation hemic and immune systems Receptors Interleukin-2 Lymphocyte Activation/drug effects Lymphocyte Function-Associated Antigen-1 T-Lymphocytes Cytotoxic/ classification/cytology/immunology/metabolism Mice Inbred C57BL Lymph Nodes/cytology Phenotype Cell Differentiation/drug effects Antigens Surface Tetradecanoylphorbol Acetate Lymph Nodes Mice Inbred C57BL/ genetics T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Immunology, Vol. 135, No 6 (1985) pp. 3704-3711 |
| ISSN: | 0022-1767 |
| Popis: | Mice bearing the recessive gene lpr develop an autoimmune syndrome associated with a massive lymphadenopathy, both of which are age and thymus dependent. The predominant accumulating cells in lymphoid tissue of lpr/lpr mice are Thy-1+ but express neither of the mature T cell markers, Lyt-2 or L3T4. We have purified this Lyt-2-/L3T4- subset and examined its phenotype. These cells are not actively cycling, do not express interleukin-2 (IL 2) receptors nor significant levels of antigen receptor, but do express the B cell marker B220. In vitro growth conditions were examined for the lpr Lyt-2-/L3T4- subset. By using a combination of phorbol ester and IL 2, these cells acquired transient expression of IL 2 receptors and grew in an IL 2-dependent manner. Furthermore, these proliferating cells underwent differentiation to a more mature T cell phenotype, with loss of cell surface B220 and acquisition, by a portion, of antigen receptor and Lyt-2. The possible parallels with normal T cell maturation are discussed. |
| Databáze: | OpenAIRE |
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