New insights into HcPTR2A and HcPTR2B, two high-affinity peptide transporters from the ectomycorrhizal model fungus Hebeloma cylindrosporum
| Autor: | Pierre-Emmanuel Courty, Tobias Müller, Gabriella Houdinet, Uwe Ludewig, Daniel Wipf, Sabine Zimmermann, Benjamin Neuhäuser |
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| Přispěvatelé: | University of Bonn, University of Hohenheim, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), German Research Foundation (DFG)DFG WI1994/2-1DFG WI1994/2-2, IZMB, Transport in Ectomycorrhiza, Rheinische Friedrich-Wilhelms-Universität Bonn |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Peptide transporter [SDV]Life Sciences [q-bio] Xenopus Plant Science 010603 evolutionary biology 01 natural sciences chemistry.chemical_compound Gene Expression Regulation Fungal Mycorrhizae Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Hebeloma Ammonium Molecular Biology Ecology Evolution Behavior and Systematics chemistry.chemical_classification biology Tripeptide transport Membrane Transport Proteins Transporter General Medicine biology.organism_classification Yeast Amino acid Ectomycorrhiza chemistry Biochemistry Heterologous expression Gene expression 010606 plant biology & botany |
| Zdroj: | Mycorrhiza Mycorrhiza, Springer Verlag, 2020, 30 (6), pp.735-747. ⟨10.1007/s00572-020-00983-7⟩ Mycorrhiza, Springer Verlag, 2020, ⟨10.1007/s00572-020-00983-7⟩ |
| ISSN: | 1432-1890 0940-6360 |
| DOI: | 10.1007/s00572-020-00983-7⟩ |
| Popis: | International audience; While plants mainly rely on the use of inorganic nitrogen sources like ammonium and nitrate, soil-borne microorganisms like the ectomycorrhizal fungus Hebeloma cylindrosporum can also take up soil organic N in the form of amino acids and peptides that they use as nitrogen and carbon sources. Following the previous identification and functional expression in yeast of two PTR-like peptide transporters, the present study details the functions and substrates of HcPTR2A and HcPTR2B by analysing their transport kinetics in Xenopus laevis oocytes. While both transporters mediated high-affinity di- and tripeptide transport, HcPTR2A also showed low-affinity transport of several amino acids-mostly hydrophobic ones with large side chains. |
| Databáze: | OpenAIRE |
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