Sequence Determination of Human Papillomavirus Type 6a and Assembly of Virus-like Particles in Saccharomyces cerevisiae
| Autor: | Cook James C, George Hugh A, Kenneth H. Fife, Mark Rosolowsky, Schultz Loren D, Kathrin U. Jansen, Darron R. Brown, Kathryn J. Hofmann, Joseph G. Joyce |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
viruses Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae Genome Viral Biology Polymerase Chain Reaction Copurification Open Reading Frames Virology Humans Point Mutation Amino Acid Sequence Cloning Molecular ORFS Codon Papillomaviridae Peptide sequence DNA Primers chemistry.chemical_classification Base Sequence Genetic Variation Puerperal Disorders biology.organism_classification Molecular biology Yeast Amino acid Open reading frame Capsid chemistry Condylomata Acuminata DNA Viral Female Vulvar Diseases |
| Zdroj: | Virology. 209(2):506-518 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1995.1283 |
| Popis: | Human papillomavirus 6a (HPV6a), the most abundant HPV6 subtype, was detected in a vulvar condyloma acuminatum. The complete genome of HPV6a was cloned, and its DNA sequence was shown to be over 97% identical to the HPV6b sequence. Of the eight open reading frames (ORFs) of HPV6a, only the imputed amino acid sequence of the major capsid protein L1 was identical to the corresponding HPV6b sequence; all other HPV6a ORFs showed amino acid changes compared to the HPV6b ORFs. The HPV6a L1 or the L1 + L2 ORFs were expressed in the yeast Saccharomyces cerevisiae. Self-assembly of the L1 capsid protein into virus-like particles (VLPs) was demonstrated both in the L1 as well as L1 + L2 coexpressing yeast strains. Copurification of the L1 and L2 proteins showed complex formation of the L1 and L2 proteins in the yeast-derived VLPs of coexpressing strains. |
| Databáze: | OpenAIRE |
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