Antibody-modified conduits for highly selective cytokine elimination from blood
| Autor: | Minh T Nguyen, Krithika Ramchander, Nikolay V. Vasilyev, Morgan Moroi, J. Brian McAlvin, Alexander M Lieber, Jason Yang, Andre Shomorony, Rohit Karnik, Obiajulu S. Okonkwo, Ryan G. Wylie, Charles K. Lok, Patrick A. Armstrong, Daniel S. Kohane |
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| Rok vydání: | 2018 |
| Předmět: |
Lipopolysaccharides
Male Vascular Endothelial Growth Factor A medicine.medical_treatment Interleukin-1beta Ischemia Inflammation Antibodies Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Immune system In vivo medicine Animals Humans 030212 general & internal medicine business.industry Tumor Necrosis Factor-alpha 030208 emergency & critical care medicine General Medicine medicine.disease Recombinant Proteins Rats Vascular endothelial growth factor A Cytokine Immunology Cytokines Tumor necrosis factor alpha medicine.symptom business Reperfusion injury Research Article |
| Zdroj: | JCI insight. 3(13) |
| ISSN: | 2379-3708 |
| Popis: | Cytokines play an important role in dysregulated immune responses to infection, pancreatitis, ischemia/reperfusion injury, burns, hemorrhage, cardiopulmonary bypass, trauma, and many other diseases. Moreover, the imbalance between inflammatory and antiinflammatory cytokines can have deleterious effects. Here, we demonstrated highly selective blood-filtering devices - antibody-modified conduits (AMCs) - that selectively eliminate multiple specific deleterious cytokines in vitro. AMCs functionalized with antibodies against human vascular endothelial growth factor A or tumor necrosis factor α (TNF-α) selectively eliminated the target cytokines from human blood in vitro and maintained them in reduced states even in the face of ongoing infusion at supraphysiologic rates. We characterized the variables that determine AMC performance, using anti-human TNF-α AMCs to eliminate recombinant human TNF-α. Finally, we demonstrated selective cytokine elimination in vivo by filtering interleukin 1 β from rats with lipopolysaccharide-induced hypercytokinemia. |
| Databáze: | OpenAIRE |
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