Long-term skin preservation by combined use of tissue culture and freezing techniques
| Autor: | Edward C. Robb, Alice D. Harper, P. Nathan, Donald L. Ballantyne |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors Cell Survival Combined use Biology Transplantation Autologous General Biochemistry Genetics and Molecular Biology Andrology Tissue culture Culture Techniques Cryoprotective Agent Freezing medicine In vitro tissue culture Animals Dimethyl Sulfoxide Frozen tissue Skin Rabbit (nuclear engineering) General Medicine Skin Transplantation Transplantation Female Frozen storage Rabbits Tissue Preservation General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 15(2) |
| ISSN: | 0011-2240 |
| Popis: | Simple but effective methods for shipping newly excised rabbit skin to a distant central laboratory for in vitro culture on a pigskin base, followed by freezing in a cryoprotective agent (DMSO) for frozen storage and subsequent reshipment to the originating laboratory while still frozen are described. At a suitable time the frozen tissue was rapidly thawed and transplanted to the autologous recipient rabbit. Of 12 cultures, seven indicated good to excellent cell growth and maturation on the host. The successful method combined the use of in vitro tissue culture and freezing to permit a central laboratory to grow the skin and ship it to the originating center for autografting at a convenient time. |
| Databáze: | OpenAIRE |
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