Myristoylated src-oncogene products are potently detected by the monoclonal antibody to the NH2-terminal myristoyl-Gly-Ser-Ser-Lys src-peptide
| Autor: | Yoko Kida, Osamu Takenaka, Yukiho Kubota, Shozo Shoji, Takayuki Funakoshi, Takashi Yoshinaga |
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| Rok vydání: | 1990 |
| Předmět: |
animal structures
medicine.drug_class Immunoprecipitation Blotting Western Molecular Sequence Data Biophysics Peptide Biology Adenocarcinoma Monoclonal antibody Biochemistry Myristic Acid Oncogene Protein pp60(v-src) Antibody Specificity medicine Tumor Cells Cultured Humans Amino Acid Sequence Molecular Biology Myristoylation chemistry.chemical_classification Rous sarcoma virus Tetrapeptide Antibodies Monoclonal Cell Biology biology.organism_classification Molecular biology Precipitin Tests Pancreatic Neoplasms chemistry embryonic structures Colonic Neoplasms biology.protein lipids (amino acids peptides and proteins) Antibody Multiple Myeloma Peptides Myristic Acids Oligopeptides Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Biochemical and biophysical research communications. 170(2) |
| ISSN: | 0006-291X |
| Popis: | Monoclonal antibodies were raised against a synthetic NH2-terminal myristoyl tetrapeptide (N-myristoyl-Gly-Ser-Ser-Lys) which is characteristic of an NH2-terminal portion of pp60src, the transforming protein of src-oncogene. The antibody reacted with the albumin conjugated with both the N-myristoyl and N-lauroyl-tetrapeptides, but concentrations at which 50% of the immunoreaction was inhibited were 5 pmol for the N-myristoyl and 830 pmol for N-lauroyl tetrapeptidyl albumin. On the other hand, N-palmitoyl tetrapeptidyl and underivatized albumin, and Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys octapeptide had no effects. These results suggest a high affinity of the antibody for an N-myristoyl-Gly-Ser-Ser-Lys moiety. src-Oncogene products in Rous sarcoma virus-transformed cells and human colon carcinoma tumor cells were selectively identified as myristoylated pp60src by immunoprecipitation analyses with the antibody. |
| Databáze: | OpenAIRE |
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