| Popis: |
Bacteriophages are the most abundant biological entity on the planet and are immensely diverse, yet little is known about them. As more phages are sequenced, their genomic characterization allows for a more comprehensive understanding of their ecological roles and genomic similarities. This study investigated the plaque assay-based isolation, characterization, and genomic analysis of soil-dwelling Arthrobacter globiformis novel phage, YesChef. Post-isolation, plaque assays revealed a temperate lifecycle and transmission electron micrographs identified YesChef’s Siphoviridae morphology. Genome sequencing and annotation placed YesChef in Cluster AZ. Clusters are groups of bacteriophages with similar genes, life cycles, and hosts and SEA-PHAGES has specific criteria to cluster phages. Genomic analyses of intercluster relationships identified cluster EH, a Microbacterium cluster, as closely related to cluster AZ. Further genomic analyses identified high similarity subgroupings within cluster AZ based on higher gene and nucleotide content, % GC content, and pham similarity. Phams are groups of similar phage genes. Phages YesChef, Powerpuff, and Lego formed a high similarity subgroup and interestingly were all collected from the West Los Angeles area. Pham similarity, cluster core genome and high similarity groupings supported taxonomic analyses and identified conserved cassettes and synteny within clusters. Collectively, these findings lay a foundation for a new way to genomically analyze phage evolution and diversity, and, thus, the further exploration and characterization of phage in the scientific world. |
