The α1-antitrypsin/elastase Complex as an Experimental Model for Hemodialysis in Acute Catabolic Renal Failure, Extracorporeal Blood Circulation and Cardiocirculatory Bypass
| Autor: | V. Grano, Umberto Bencivenga, N. Diano, N. G. De Santo, Franca Salamino, A. De Maio, Damiano Gustavo Mita, Marianna Portaccio, Alessandra F. Perna |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Extracorporeal Circulation
medicine.medical_specialty Proteases Autolysis (biology) Polymers medicine.medical_treatment 030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) Bioengineering In Vitro Techniques 030204 cardiovascular system & hematology Extracorporeal law.invention Biomaterials 03 medical and health sciences 0302 clinical medicine Renal Dialysis law Internal medicine medicine Cardiopulmonary bypass Protease Inhibitors Sulfones Cardiopulmonary Bypass Protease business.industry Serine Endopeptidases Elastase Membranes Artificial General Medicine Acute Kidney Injury Enzymes Immobilized Membrane Endocrinology alpha 1-Antitrypsin Cardiology Hemodialysis Carrier Proteins Leukocyte Elastase business |
| Zdroj: | 25 (2002): 297–305. info:cnr-pdr/source/autori:Grano, V; Diano, N; Portaccio, M; Bencivenga, U; De Maio, A; De Santo, N; Perna, A; Salamino, F; Mita, DG/titolo:The alpha(1)-antitrypsin%2Felastase complex as an experimental model for hemodialysis in acute catabolic renal failure, extracorporeal blood circulation and cardiocirculatory bypass/doi:/rivista:/anno:2002/pagina_da:297/pagina_a:305/intervallo_pagine:297–305/volume:25 Europe PubMed Central |
| ISSN: | 1724-6040 0391-3988 |
| DOI: | 10.1177/039139880202500408 |
| Popis: | A modified polyethersulphone graft membrane was loaded with antiproteases, with the aim of reducing the active protease blood concentration during hemodialysis in acute catabolic renal failure or cardiopulmonary bypass. As protease/antiprotease system, elastase and α1-antitrypsin were used. The concentration of active elastase in aqueous solutions decreased as function of contact time with the membrane, approaching saturation. A 40% loss of elastase activity was obtained at pH 7.4, which was not due to autolysis, which accounted for 5% of the loss. The highest reduction was achieved at pH 9.0 (25% higher than at pH 7.4). The saturation level of elastase decrease, calculated by means of the Einstein equation, was reached after more than 47 minutes. We speculate that a time reduction might be achieved either increasing the concentration of immobilized antiproteases, or increasing the rate of elastase movement across the membranes by hydraulic, osmotic, or temperature gradients. This technology can be applied to hemodialysis, and in extracorporeal blood circulation to promote elastase release. |
| Databáze: | OpenAIRE |
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