Optimizing bone wound healing using BMP2 with absorbable collagen sponge and Talymed nanofiber scaffold
| Autor: | Martin Steed, Amanda C. LaRue, Brayden Oakes, James J. Cray, SarahRose Hall, Zachary Grey, R. Nicole Howie, Reed Houck, Nicholas Larson, Robin C. Muise-Helmericks, Emily Durham |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Scaffold animal structures Nanofibers BMP2 Bone Morphogenetic Protein 2 lcsh:Medicine Bioinformatics Bone morphogenetic protein 2 Ectopic bone formation General Biochemistry Genetics and Molecular Biology Bone tissue engineering Biomaterials 03 medical and health sciences Tissue engineering Medicine Animals Wound Healing Tissue Scaffolds business.industry Research fungi Skull lcsh:R Nanofiber scaffold General Medicine X-Ray Microtomography 3. Good health Mice Inbred C57BL 030104 developmental biology Collagen sponge embryonic structures Female Collagen business Wound healing Bone wound healing |
| Zdroj: | Journal of Translational Medicine, Vol 16, Iss 1, Pp 1-8 (2018) Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Popis: | Background Bone is a highly vascularized and resilient organ with innate healing abilities, however some bone injuries overwhelm these attributes and require intervention, such as bone tissue engineering strategies. Combining biomaterials and growth factors, such as bone morphogenetic protein 2 (BMP2), is one of the most commonly used tissue engineering strategies. However, use of BMP2 has been correlated with negative clinical outcomes including aberrant inflammatory response, poor quality bone, and ectopic bone. Methods In the present study, a novel poly-n-acetyl glucosamine (pGlcNAc, trade name Talymed) scaffold was utilized in addition to the commonly used acellular collagen sponge (ACS) BMP2 delivery system in a murine calvarial defect model to investigate whether the innate properties of Talymed can reduce the noted negative bone phenotypes associated with BMP2 treatment. Results Comparison of murine calvarial defect healing between ACS with and without Talymed revealed that there was no measurable healing benefit for the combined treatment. Healing was most effective utilizing the traditional acellular collagen sponge with a reduced dose of BMP2. Conclusions The results of this investigation lead to the conclusion that excessive dosing of BMP2 may be responsible for the negative clinical side effects observed with this bone tissue engineering strategy. Rather than augmenting the currently used ACS BMP2 bone wound healing strategy with an additional anti-inflammatory scaffold, reducing the dose of BMP2 used in the traditional delivery system results in optimal healing without the published negative side effects of BMP2 treatment. |
| Databáze: | OpenAIRE |
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