Construction of representative NotI linking libraries specific for the total human genome and for human chromosome 3

Autor:	Vladimir M. Bannikov, Lev L. Kisselev, Michael Dean, Eugene R. Zabarovsky, Rando Allikmets, George Klein, Vladimir I. Kashuba, Irina Kholodnyuk, Niklas Paulsson, Veronika I. Zabarovska
Rok vydání:	1994
Předmět:	Genetics Genetic Linkage Genome Human Chromosome Biology Genome Restriction enzyme Blotting Southern Plasmid Gene mapping Chromosome 3 Humans Genomic library Human genome Chromosomes Human Pair 3 Cloning Molecular Deoxyribonucleases Type II Site-Specific Gene Library
Zdroj:	Genomics. 20(2)
ISSN:	0888-7543 3000-5000
Popis:	NotI linking clones represent valuable tools for both physical and genetic mapping. Using procedures that we have previously described, several chromosome 3-specific NotI linking libraries have been constructed. Here, we describe the construction of six independent NotI linking libraries specific for the total human genome. These libraries were made using three different vectors and two combinations of restriction enzymes. Altogether, these six libraries contain more than 1 million recombinant phages. Considering that the human genome contains about 3000-5000 NotI sites, it is likely that all clonable NotI sites are present in these libraries. Two of the six libraries were transferred into plasmid form. At the same time, a chromosome 3-specific EcoRI-NotI library (NRL1) was constructed. This library considerably increases the representation of cloned NotI sites in combination with previously constructed libraries that were made using BamHI-NotI digestion. All libraries are available on request.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00bb5ec4145a9116b07fbff86ec1abcc https://pubmed.ncbi.nlm.nih.gov/8020985 Zobrazit plný text záznamu