Determination of glycogen and enzymes of glycogen metabolism in human hair follicles
| Autor: | Charles Sultan, Alphons J.M. Vermorken, A. M. G. Vermeesch-Markslag, C. M. A. A. Goos, J. W. J. van der Stappen, A. H. G. M. Beaumont |
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| Rok vydání: | 1987 |
| Předmět: |
Phosphorylases
Phosphorylase Kinase Biology Glycogen phosphorylase chemistry.chemical_compound Genetics medicine Humans Phosphorylase a Phosphorylase b Phosphorylase kinase Molecular Biology Cells Cultured hirsutism integumentary system Glycogen Microchemistry DNA General Medicine Metabolism Hair follicle medicine.disease Epithelium medicine.anatomical_structure Biochemistry chemistry Energy source Hair |
| Zdroj: | Molecular Biology Reports. 12:259-264 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/bf00444677 |
| Popis: | The skin epithelium and its organelles use glycogen as well as glucose as source of energy. Therefore the characterisation of glycogen metabolism and the enzymes involved is important in the study of mechanisms regulating the normal or abnormal differentiation of skin organelles such as sebaceous glands and hair follicles. The present paper describes fluorimetric methods for the determination of glycogen and for the measurements of phosphorylase and phosphorylase kinase activity in one and the same lysate of minute tissue samples. The methods were tested for their suitability on freshly isolated human hair follicles and cultured hair follicle cells. The possible use of these techniques for studies on the pathophysiology of acne and hirsutism is discussed. |
| Databáze: | OpenAIRE |
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