Antigen-induced elevation of immunoreactive endothelin-1 (ET-1) levels in ovalbumin-sensitized guinea pig airway tissue
| Autor: | Hajime Murakami, Tadashi Kusama, Kiyoshige Saito, Hiroyuki Kotake, Yasuo Kizawa |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Ovalbumin Guinea Pigs Immunology Epithelium Guinea pig chemistry.chemical_compound Antigen Internal medicine medicine Animals Antigens Anaphylaxis Pharmacology Endothelin-1 biology Chemistry Phosphoramidon Glycopeptides Anaphylactic reaction Muscle Smooth respiratory system Immunohistochemistry Endothelin 1 Trachea medicine.anatomical_structure Endocrinology biology.protein Airway |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0742-8413 |
| DOI: | 10.1016/s0742-8413(98)10122-6 |
| Popis: | Changes in the immunoreactive ET-1 levels during the anaphylactic reaction of airway tissue from ovalbumin-sensitized guinea pigs were investigated. ET-1-immunoreactivity (ET-IR) was detected in the epithelial and smooth muscle layers of tracheal sections from normal guinea pigs and it was enhanced slightly by phosphoramidon (1 microM) treatment. The ET-IR level of the epithelial layer of ovalbumin-treated tissue from actively sensitized animals was slightly higher than that from normal animals, but it was enhanced markedly by phosphoramidon (1 microM) treatment. Furthermore, the mean ET-IR level of homogenates of antigen-treated tracheal tissues from sensitized guinea pigs (22.8 +/- 1.55 fmol mg-1 protein, n = 5) was significantly higher than the corresponding normal level (12.3 +/- 1.21 fmol mg-1 protein, n = 5). These results suggest that increased epithelial airway ET-1 levels contribute to the anaphylactic reaction of guinea pig airways. |
| Databáze: | OpenAIRE |
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