Partial characterization of a glycosylphosphatidyl-inositol (GPI) in porcine thyroid cultured cells
| Autor: | Hervé Sartelet, Bernard Haye, Emmanuelle Petitfrère, Laurent Martiny, G. Aguie-Aguie |
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| Rok vydání: | 1993 |
| Předmět: |
Chromatography
Gas Glycosylphosphatidylinositols Swine Thyroid Gland Deamination Phospholipid Nitrous Acid Oleic Acids Biology Phosphatidylinositols Tritium Biochemistry Phosphates chemistry.chemical_compound Hydrolysis Glucosamine Animals Inositol Cells Cultured Diacylglycerol kinase chemistry.chemical_classification Glycosidic bond General Medicine Oleic acid chemistry lipids (amino acids peptides and proteins) Hydrochloric Acid Oleic Acid |
| Zdroj: | Biochimie. 75:427-431 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/0300-9084(93)90107-4 |
| Popis: | The aim of this study was to provide information on the structure of glycosylphosphatidylinositol (GPI) and to characterize this novel phospholipid isolated from pig thyroid. We investigated the incorporation of different radioactive precursors: [3H]glucosamine, [3H]inositol, [3H]oleic acid, [32P]orthophosphate and demonstrated the presence of these moieties in the structure of GPI. After labelling and hydrochloric acid hydrolysis, the major part of radioactivity comigrated with glucosamine used as marker. Cleavage of GPI by nitrous acid deamination indicated the presence of a glycosidic bond between the lipid moiety and glucosamine. The fatty acid composition of diacylglycerol was also studied by gas chromatography. Oleic acid was found preferentially to other fatty acids. In a previous paper we reported that a chronic treatment with 0.1 mU/ml thyrotropin (TSH) of thyroid cultured cells for 3 days produced a large increase in the turnover rate of GPI and concomitantly the release of a water-soluble product of GPI hydrolysis: an inositolphosphateglycan (IPG). These findings confirm that GPI may play a role in membrane signalling systems and thyroid cell regulation. |
| Databáze: | OpenAIRE |
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