Novel CIPK1-Associated Proteins in Arabidopsis Contain an Evolutionarily Conserved C-Terminal Region That Mediates Nuclear Localization
Autor: | Hye Jin Jeong, Sheng Luan, Kyung Nam Kim, Sung Han Ok, Jeong Sheop Shin, Jung Myung Bae |
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Rok vydání: | 2005 |
Předmět: |
Physiology
Molecular Sequence Data Arabidopsis Plant Science Protein Serine-Threonine Kinases Biology Evolution Molecular Gene Expression Regulation Plant Gene expression Genetics medicine Arabidopsis thaliana Amino Acid Sequence Calcium Signaling Gene Conserved Sequence Cell Nucleus Regulation of gene expression Sequence Homology Amino Acid Arabidopsis Proteins Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins biology.organism_classification Cell biology Protein Transport medicine.anatomical_structure Cytoplasm Nucleus Nuclear localization sequence Protein Binding Research Article |
Zdroj: | Plant Physiology. 139:138-150 |
ISSN: | 1532-2548 0032-0889 |
Popis: | Environmental stimuli, including light, pathogens, hormones, and abiotic stresses, elicit changes in the cytosolic Ca2+ signatures of plant cells. However, little is known about the molecular mechanisms by which plants sense and transmit the specific cytoplasmic Ca2+ signal into the nucleus, where gene regulation occurs to respond appropriately to the stress. In this study, we have identified two novel Arabidopsis (Arabidopsis thaliana) proteins specifically associated with Calcineurin B-Like-Interacting Protein Kinase1 (CIPK1), a member of Ser/Thr protein kinases that interact with the calcineurin B-like Ca2+-binding proteins. These two proteins contain a very similar C-terminal region (180 amino acids in length, 81% similarity), which is required and sufficient for both interaction with CIPK1 and translocation to the nucleus. Interestingly, the conserved C-terminal region was also found in many proteins from various eukaryotic organisms, including humans. However, none of them have been characterized so far. Taken together, these findings suggest that the two proteins containing the evolutionarily conserved C-terminal region (ECT1 and ECT2) may play a critical role in relaying the cytosolic Ca2+ signals to the nucleus, thereby regulating gene expression. |
Databáze: | OpenAIRE |
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