Typing of Mycobacterium tuberculosis strains isolated in Community Health Centers of Rio de Janeiro City, Brazil
| Autor: | Afranio Lineu Kritski, Amanda Nogueira Brum Fontes, Yolanda Bravin, Wim Degrave, Rossana Coimbra Brito, G. Silva, Anna Cristina Calçada Carvalho, Marcia Aparecida da Silva Pereira, Philip Noel Suffys, Maraníbia Cardoso Oelemann |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Adult Male medicine.medical_specialty lcsh:Arctic medicine. Tropical medicine Tuberculosis Genotype double repetitive element lcsh:RC955-962 lcsh:QR1-502 IS6110-restriction fragment length polymorphism Disease cluster molecular epidemiology lcsh:Microbiology Mycobacterium tuberculosis Risk Factors Environmental health Epidemiology medicine Cluster Analysis Humans Typing Prospective Studies Molecular epidemiology biology business.industry Incidence (epidemiology) Community Health Centers Middle Aged medicine.disease biology.organism_classification DNA Fingerprinting Bacterial Typing Techniques tuberculosis Female Restriction fragment length polymorphism business Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 102, Issue: 4, Pages: 455-462, Published: 03 MAY 2007 Memórias do Instituto Oswaldo Cruz., Vol 102, Iss 4, Pp 455-462 (2007) |
| Popis: | Fingerprinting of Mycobacterium tuberculosis strains from tuberculosis (TB) patients attended in Community Health Centers (CHCs) of Rio de Janeiro was performed to verify possible risk factors for TB transmission. A prospective community-based study was performed during the period of July 1996 to December 1996 by collecting sputum samples of 489 patients in 11 different CHCs in four different planning areas (APs) of the city. Bacteriological, clinical, and epidemiological information was collected and M. tuberculosis genotypes defined after restriction fragment length polymorphism (IS6110-RFLP) and double repetitive element (DRE) fingerprinting of RFLP-clustered cases. Risk factors for TB transmission were looked for using three levels of cluster stringency. Among 349 (71%) positive cultures obtained, IS6110-RFLP typing could be performed on strains from 153 different patients. When using identity of RFLP patterns as cluster definition, 49 (32%) of the strains belonged to a cluster and none of the clinical or epidemiologic characteristics was associated with higher clustering levels. However, higher clustering level was observed in the AP including the central region of the city when compared to others. This strongly suggests that more recent transmission occurs in that area and this may be related with higher incidence of TB and HIV in this region. |
| Databáze: | OpenAIRE |
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