Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

Autor: Muller, Christian D., Minker, Carole, Duban, Livine, Karas, Daniel, Järvinen, Päivi, Lobstein, Annelise, Muller, Christian
Přispěvatelé: Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Immunité et cancer (U932), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Curie-Université Paris Descartes - Paris 5 (UPD5), Laboratoire d'Innovation Thérapeutique (LIT), Cooltech Applications, Cooltech, Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Aging
Colorectal cancer
Cell
Blueberry Plants
Apoptosis
Biochemistry
p38 Mitogen-Activated Protein Kinases
TNF-Related Apoptosis-Inducing Ligand
0302 clinical medicine
Phosphorylation
ComputingMilieux_MISCELLANEOUS
0303 health sciences
Caspase 8
biology
Caspase 3
lcsh:Cytology
General Medicine
Caspase 9
Lowbush blueberry
medicine.anatomical_structure
030220 oncology & carcinogenesis
Colonic Neoplasms
DNA fragmentation
Research Article
Article Subject
[SDV.CAN]Life Sciences [q-bio]/Cancer
DNA Fragmentation
Phosphatidylserines
Cell Line
03 medical and health sciences
medicine
Humans
Proanthocyanidins
Vaccinium vitis-idaea
fas Receptor
lcsh:QH573-671
030304 developmental biology
Plant Extracts
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
Cell Biology
DNA
biology.organism_classification
medicine.disease
Receptors
TNF-Related Apoptosis-Inducing Ligand
Drug Resistance
Neoplasm
Fruit
Cancer cell
Cancer research
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Zdroj: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Hindawi, 2015, 2015, pp.1-13. ⟨10.1155/2015/154164⟩
Oxidative Medicine and Cellular Longevity, Vol 2015 (2015)
ISSN: 1942-0900
1942-0994
DOI: 10.1155/2015/154164⟩
Popis: Scope. The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.Methods and Results. Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.Conclusion. We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.
Databáze: OpenAIRE
Externí odkaz: