The Cardioprotective Effect of Icariin on Ischemia-Reperfusion Injury in Isolated Rat Heart: Potential Involvement of the PI3K-Akt Signaling Pathway

Autor:	Ai-Mei Gao, Lijuan Ji, Zun-Ping Ke, Peng Xu, Lei Wang, Jingjing Liu
Rok vydání:	2015
Předmět:	Male Ischemia Apoptosis Myocardial Reperfusion Injury Pharmacology medicine.disease_cause Phosphatidylinositol 3-Kinases Random Allocation chemistry.chemical_compound Malondialdehyde Lactate dehydrogenase medicine Animals Creatine Kinase MB Form Pharmacology (medical) PI3K/AKT/mTOR pathway Flavonoids Cardioprotection L-Lactate Dehydrogenase Superoxide Dismutase business.industry Heart General Medicine medicine.disease Rats Oxidative Stress chemistry Anesthesia Heart Function Tests cardiovascular system Cardiology and Cardiovascular Medicine business Icariin Reperfusion injury Oxidative stress
Zdroj:	Cardiovascular Therapeutics. 33:134-140
ISSN:	1755-5914
DOI:	10.1111/1755-5922.12121
Popis:	SummaryAims Icariin (ICA), a flavonoid isolated from epimedii, has been reported to have potential protective effects on the cardiovascular system. This study is to investigate the effect and the underlying mechanisms of ICA on ischemia/reperfusion (I/R) injury. Methods Wister rat hearts were subjected to I/R using Langendorff perfusion system. Cardiac function, myocardial infarct size, lactate dehydrogenase (LDH), and creatine kinase-MB (CK-MB) activities in coronary effluent, and superoxide dismutase (SOD) and malondialdehyde (MDA) content in heart tissue and cardiomyocyte apoptosis were assayed. Results Compared with the I/R group, ICA treatment significantly improved cardiac function, decreased myocardial infarct size, enzyme activity, oxidative stress, and apoptosis. In addition, ICA treatment lead to an increased p-Akt level, which was partially reversed by LY294002, a PI3K pathway inhibitor. Conclusion Our study suggests that ICA has a cardioprotective effect against I/R injury, which is associated with its antioxidative and anti-apoptotic effect, at least partially, through the activation of PI3K-Akt signaling pathway.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00a6a0f34c53a62bbb474822877e00cc https://doi.org/10.1111/1755-5922.12121 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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