Development of an enteric-coated, layered multi-particulate formulation for ileal delivery of viable recombinant Lactococcus lactis
| Autor: | N HUYGHEBAERT, A VERMEIRE, P ROTTIERS, E REMAUT, J REMON |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
food.ingredient
Chemistry Pharmaceutical Drug Storage Inulin Pellets Pharmaceutical Science Dosage form Microbiology chemistry.chemical_compound food Drug Delivery Systems Drug Stability Polymethacrylic Acids Ileum Skimmed milk medicine Technology Pharmaceutical Food science Lactose biology Lactococcus lactis General Medicine biology.organism_classification Enteric coating Interleukin-10 chemistry Tablets Enteric-Coated Drug carrier Biotechnology medicine.drug |
| Zdroj: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 61(3) |
| ISSN: | 0939-6411 |
| Popis: | Layering of recombinant hIL-10 producing Lactococcus lactis (L. lactis Thy12) on inert carriers is a promising technique for the preparation of a multi-particulate formulation of viable, hIL-10 producing L. lactis. To improve viability after layering and storage, L. lactis Thy12 was layered in different matrices (10% skim milk and/or 2.5, 5, 10% inulin). After layering, the highest viability was obtained in the 10% skim milk supplemented with 5% inulin matrix (8.7%). However, upon storage, 10% skim milk alone yielded the highest viability. Thereby, layered L. lactis Thy12 showed superior long term stability in comparison with freeze-dried L. lactis Thy12. The layering process was performed during 3h without encountering technical problems, with good layer consistence and constant viability. Enteric properties were obtained with a 30% Eudragit L30D-55 or 15% Eudragit FS30D coating and maintained during an initial six months storage period (-20 degrees C/20% RH). After in vitro simulation of the gastric stage, only 5% of the bacteria remained viable in Eudragit L30D-55 coated pellets, contrary to 85% in Eudragit FS30D coated pellets, indicating its superior protective capacity against gastric fluid. After eight months storage (-20 degrees C), 80% of the initial L. lactis Thy12 remained viable in the Eudragit FS30D coated pellets. |
| Databáze: | OpenAIRE |
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