Nestin expression in Müller glial cells in postnatal rat retina and its upregulation following optic nerve transection
| Autor: | Qiong Cao, Jia Lu, L.P. Xue, Charanjit Kaur, Eng-Ang Ling |
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| Rok vydání: | 2006 |
| Předmět: |
Cell Count
Nerve Tissue Proteins macromolecular substances Retina Nestin Intermediate Filament Proteins Glutamate-Ammonia Ligase Glial Fibrillary Acidic Protein medicine Animals RNA Messenger Rats Wistar Cellular localization Cells Cultured Glial fibrillary acidic protein biology Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Age Factors Gene Expression Regulation Developmental GFAP stain Immunohistochemistry Cell biology Rats medicine.anatomical_structure nervous system Animals Newborn Optic Nerve Injuries Phosphopyruvate Hydratase biology.protein Optic nerve Neuroglia sense organs NeuN Neuroscience |
| Zdroj: | Neuroscience. 143(1) |
| ISSN: | 0306-4522 |
| Popis: | This study examined the nestin immunoexpression and its specific cellular localization in the developing retina of rats and investigated its putative changes in an altered environment. At postnatal day 0, nestin immunoexpression was detected in radially oriented cells considered to be neural progenitors that were glutamine synthetase (GS) negative. With age, it was localized in differentiating and differentiated GS positive Muller glial cells. Nestin expression was down-regulated as maturation proceeded, so that by 12 weeks, it was almost completely diminished as confirmed also by real time-polymerase chain reaction analysis. Nestin expression along with that of glial fibrillary acidic protein (GFAP) was induced and upregulated in mature Muller glial cells following optic nerve transection. It is suggested that both nestin and GFAP may be useful biomarkers in retinal injuries. In view of their cytoskeletal nature, the marked expression of nestin and GFAP may provide a structural support for the framework of retina which would be disrupted as a result of loss of neurons in optic nerve lesion. It may also be neuronal protective taking into consideration the close spatial and functional links between Muller glial cells and the axotomized ganglion cells. |
| Databáze: | OpenAIRE |
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