Specific detection of p-chlorobenzoic acid by Escherichia coli bearing a plasmid-borne fcbA':: lux fusion
| Autor: | Karl-Heinz Gartemann, Ali Nejidatl, Shimshon Belkin, Yael Rozen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Environmental Engineering
Operon Hydrolases Health Toxicology and Mutagenesis Industrial Waste Environmental pollution medicine.disease_cause Benzoates Plasmid Arthrobacter medicine Escherichia coli Environmental Chemistry Dehalogenase biology Photobacterium Public Health Environmental and Occupational Health General Medicine General Chemistry 4-chlorobenzoate dehalogenase Hydrogen-Ion Concentration biology.organism_classification Pollution Culture Media Chlorobenzoates Biochemistry Luminescent Measurements Light emission Indicators and Reagents Plasmids |
| DOI: | 10.1016/s0045-6535(98)00210-0 |
| Popis: | In this communication we report on a genetically engineered bacterium that reacts by light emission to the presence of 4-chlorobenzoic acid. To construct this strain, DNA fragment (1.7 kb) upstream from the 4-chlorobenzoic acid dehalogenase (fcb) operon of Arthrobacter SU was fused to Vibrio fischeri luxCDABE genes. An Escherichia coli strain transformed with a multi-copy plasmid (pASU) bearing this fusion responded to the presence of 4-chlorobenzoic acid and a few closely related compounds by increased luminescence, exhibiting a high specificity but a relatively low sensitivity. While it could be somewhat, improved by manipulating the experimental pH, sensitivity remained too low for real time applicability. Nevertheless, the principle of using dehalogenase promoters as environmental pollution sensor was demonstrated. (C) 1998 Elsevier Science Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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