IL-37b suppresses T cell priming by modulating dendritic cell maturation and cytokine production via dampening ERK/NF-κB/S6K signalings
| Autor: | Yun Wang, Hanzhi Liu, Weiqiang Wang, Zhongchao Han, Yue Shen, Song Chen, Wenwen Li, Ling Han, Kui Dong, Xiaoming Feng, Gang Yu, Zhina Sun, Juan Yu, Lijun Fang, Wantong Wu, Zhonglong Li, Chunmin Fang, Yuechen Luo |
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| Rok vydání: | 2015 |
| Předmět: |
Male
MAPK/ERK pathway MAP Kinase Signaling System T-Lymphocytes Cellular differentiation medicine.medical_treatment T cell Biophysics Priming (immunology) chemical and pharmacologic phenomena Biology Biochemistry Mice Cross-Priming Suppressor Factors Immunologic medicine Animals CD86 Interleukins NF-kappa B Ribosomal Protein S6 Kinases 70-kDa Cell Differentiation Dendritic Cells General Medicine Dendritic cell Recombinant Proteins Cell biology Mice Inbred C57BL Cytokine medicine.anatomical_structure Immunology Cytokines CD80 |
| Zdroj: | Acta Biochimica et Biophysica Sinica. 47:597-603 |
| ISSN: | 1672-9145 |
| DOI: | 10.1093/abbs/gmv058 |
| Popis: | Interleukin 37b (IL-37b) plays a key role in suppressing immune responses, partially by modulating the function of dendritic cells (DCs). However, the precise mechanisms are still largely unknown. Here, we investigated the effects of IL-37b on DC maturation and T cell responses induced by DCs, and explored the involved signaling pathways. It was found that IL-37b down-regulated the expressions of co-stimulatory molecules CD80 and CD86 on DCs in vitro. At the same time, the expressions of pro-inflammatory cytokines, such as TNF-α and IL-6, were suppressed, while the expression of the T cell inhibitory cytokine TGF-β was increased in IL-37b-treated DCs. In addition, the activation effect of DCs on T cells was impaired by IL-37b. We further revealed that extracellular single-regulated kinase (ERK), nuclear factor-κB (NF-κB), and mTOR-S6K signaling pathways were involved in the inhibition of DCs induced by IL-37b. This was confirmed by the similarly suppressive effect of chemical inhibitors against NF-κB, ERK, and S6K on the expressions of IL-6 and TNF-α in DCs. In conclusion, these results demonstrated that IL-37b suppressed DC maturation and immunostimulatory capacity in T cell priming by involving in ERK, NF-κB, and S6K-based inhibitory signaling pathways. |
| Databáze: | OpenAIRE |
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