Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome
| Autor: | Hiroaki Okamoto, Haruko Miyajima, Masaharu Takahashi, Hitoshi Mizuo, Yuhko Gotanda, Tsutomu Nishizawa |
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| Rok vydání: | 2003 |
| Předmět: |
Genotype
Swine Sequence analysis viruses Molecular Sequence Data Genome Viral Biology Japan Zoonoses Virology Hepatitis E virus medicine Animals Humans Amino Acid Sequence ORFS Peptide sequence Phylogeny Swine Diseases Hepatitis Genetics Base Sequence Nucleic acid sequence Sequence Analysis DNA Middle Aged medicine.disease Hepatitis E Open reading frame Hepatitis Viral Animal |
| Zdroj: | Journal of General Virology. 84:1245-1251 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.19052-0 |
| Popis: | The full-length genomic sequences were determined of Japanese swine and human hepatitis E virus (HEV) isolates (swJ13-1 and HE-JA1, respectively) with 100 % identity in the partial sequence of open reading frame (ORF) 2 (ORF2, 412 nt). swJ13-1 was isolated from a 4-month-old farm pig born in Hokkaido, Japan, in 2002 and HE-JA1 was recovered from a 55-year-old patient who lived in Hokkaido and who had contracted sporadic acute hepatitis E in 1997. Both isolates consisted of 7240 nt, excluding the poly(A) tail, and contained three ORFs (ORFs 1-3) that encoded proteins of 1707, 674 and 114 aa. The overall nucleotide sequence identity between them was 99.0 % and the deduced amino acid sequence identities of ORFs 1-3 were 99.8, 100 and 100 %, respectively. The high degree of genomic similarity observed between swine and human HEV isolates in a restricted area of Japan further supports the finding that sporadic hepatitis E in Japan is a zoonosis. |
| Databáze: | OpenAIRE |
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