Ultrastructural and histological characteristics of the endometrium during early embryo development in mares
| Autor: | María Noel Martínez, M.I.M. Jobim, Sandra Mara Fiala-Rechsteiner, Ricardo Macedo Gregory, R.C. Mattos, Giovani Casanova Camozzato, Ana Meikle, H.B.A. Bastos |
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| Rok vydání: | 2019 |
| Předmět: |
endocrine system
animal diseases Embryonic Development Biology Endometrium Andrology 03 medical and health sciences 0302 clinical medicine Food Animals Pregnancy medicine Animals Horses Small Animals reproductive and urinary physiology Estrous cycle 030219 obstetrics & reproductive medicine urogenital system Equine Embryogenesis 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences medicine.disease 040201 dairy & animal science Ovulation Detection medicine.anatomical_structure Pregnancy Animal Female Animal Science and Zoology sense organs Sample collection Blood vessel |
| Zdroj: | Theriogenology. 123:1-10 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2018.09.018 |
| Popis: | The aim of this study was to evaluate ultrastructural and histological changes in the endometrium on days 7, 10 and 13 post-ovulation in pregnant and cyclic mares. Mares were routinely examined by transrectal palpation and ultrasonographic examination of the reproductive tract until estrus was detected. In the first cycle, endometrial biopsies from 30 cyclic mares (Cyclic group) were collected on days 7, 10 and 13 post-ovulation. In the second cycle, the same mares were bred by a fertile stallion. At days 7, 10 and 13 post-ovulation intrauterine biopsies were collected. Immediately after sample collection, the mare's uteri were flushed, and those mares with embryo recovery were assigned to the Pregnant group. From ovulation detection until day of uterine biopsy, blood samples to measure Progesterone concentrations were collected daily in cyclic and pregnant mares. A larger blood vessel caliber was observed in pregnant mares than in cyclic from day 7-13. On the 7th day of pregnancy a large loss of ciliated cells was evident in the group of pregnant mares in comparison with the Cyclic group and the superficial cells of the endometrium were more protruded, and a small amount of histotrophic material between the folds was observed. On the 10th day of pregnancy, the glandular histotrophic secretion and the secretion of luminal epithelium became more intense than the secretion of cyclic mares. On the 13th day of pregnancy, a very large amount of histotroph was observed within large glandular openings surrounded by ciliated cells. The concentrations of P4 were affected by day (P 0.001), but were not affected by group. Changes occurred in the uterine environment thereupon the entry of the embryo into the uterus. In the stroma and in the lumen, these modifications may aid to provide the necessary nutrition for the initial development of the embryo and to promote changes at cellular structures that will interact in the embryonic signaling and future fixation, implantation and placentation. |
| Databáze: | OpenAIRE |
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