AFF4 regulates osteogenic differentiation of human dental follicle cells
| Autor: | Rui Sheng, Qingyue Xiao, Ruoshi Xu, Quan Yuan, Xingying Qi, Y. Chen, Yuning Zhang, Chenchen Zhou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scaffold protein
Stem-cell differentiation Article Transcription (biology) Osteogenesis Humans Epigenetics General Dentistry Transcription factor Cells Cultured Dental follicle Messenger RNA Protein translocation Chemistry Cell Differentiation Dental Sac DLX5 Cell biology RUNX2 lcsh:RK1-715 Repressor Proteins Gene Expression Regulation lcsh:Dentistry Transcriptional Elongation Factors Biomarkers Transcription Factors |
| Zdroj: | International Journal of Oral Science International Journal of Oral Science, Vol 12, Iss 1, Pp 1-11 (2020) |
| ISSN: | 2049-3169 1674-2818 |
| Popis: | As a member of the AFF (AF4/FMR2) family, AFF4 is a transcription elongation factor that is a component of the super elongation complex. AFF4 serves as a scaffolding protein that connects transcription factors and promotes gene transcription through elongation and chromatin remodelling. Here, we investigated the effect of AFF4 on human dental follicle cells (DFCs) in osteogenic differentiation. In this study, we found that small interfering RNA-mediated depletion ofAFF4resulted in decreased alkaline phosphatase (ALP) activity and impaired mineralization. In addition, the expression of osteogenic-related genes (DLX5,SP7,RUNX2andBGLAP) was significantly downregulated. In contrast, lentivirus-mediated overexpression ofAFF4significantly enhanced the osteogenic potential of human DFCs. Mechanistically, we found that both the mRNA and protein levels of ALKBH1, a critical regulator of epigenetics, changed in accordance with AFF4 expression levels. Overexpression ofALKBH1inAFF4-depleted DFCs partially rescued the impairment of osteogenic differentiation. Our data indicated that AFF4 promoted the osteogenic differentiation of DFCs by upregulating the transcription ofALKBH1. |
| Databáze: | OpenAIRE |
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