Protection ofpsbAIITranscript from Ribonuclease Degradationin Vitroby DnaK2 and DnaJ2 Chaperones of the CyanobacteriumSynechococcus elongatusPCC 7942
| Autor: | Kaori Nimura-Matsune, Hirofumi Yoshikawa, Taku Chibazakura, Masumi Sato, Satoru Watanabe |
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| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic RNase P Endoribonuclease macromolecular substances Biology Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Bacterial Proteins Endoribonucleases HSP70 Heat-Shock Proteins Ribonuclease Molecular Biology Gene Synechococcus Messenger RNA Organic Chemistry General Medicine HSP40 Heat-Shock Proteins biology.organism_classification Molecular biology RNase MRP Chaperone (protein) biology.protein bacteria Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 71:279-282 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.60647 |
| Popis: | Three dnaK and four dnaJ genes have been identified in the genome of cyanobacterium Synechococcus elongatus PCC 7942. Our comprehensive analysis of yeast two-hybrid screening revealed a specific interaction among DnaK2, DnaJ2, and RNase E, an essential endoribonuclease. We examined the effects of DnaK2 and DnaJ2 on RNase E activity by monitoring the digestion of psbAII transcript in vitro. The addition of DnaK2 and DnaJ2 obviously inhibited RNase E activity in an ATP-dependent manner. These results suggest that DnaK2 and DnaJ2 are involved in RNA degradation through interaction with RNase E. |
| Databáze: | OpenAIRE |
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