In Utero Exposure to Di(n-butyl)phthalate Induces Morphological and Biochemical Changes in Rats Postpuberty
| Autor: | Hiroyuki Takahashi, Mitsuru Sugiyama, Masaya Motohashi, Tomoko Mutou, Hiroshi Ikegami, Shin Wakui, Yuya Okayama, Eisuke Kume, Michael F. Wempe |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Offspring Biology Toxicology Pathology and Forensic Medicine Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy Internal medicine Testis medicine Animals Testosterone Sexual Maturation Molecular Biology Sertoli Cells urogenital system Phthalate Organ Size Cell Biology Seminiferous Tubules Sertoli cell Dibutyl Phthalate Rats 030104 developmental biology Endocrinology Seminiferous tubule medicine.anatomical_structure Animals Newborn chemistry Maternal Exposure In utero Prenatal Exposure Delayed Effects Gestation Female Follicle Stimulating Hormone 030217 neurology & neurosurgery Hormone |
| Zdroj: | Toxicologic Pathology. 45:526-535 |
| ISSN: | 1533-1601 0192-6233 |
| DOI: | 10.1177/0192623317709091 |
| Popis: | Pregnant Sprague-Dawley rats were orally administered di( n-butyl)phthalate (DBP; 100 mg/kg/day) on gestation days (GD) 12 to 21. We investigated the male offspring and probed morphological alterations in Sertoli cells at 7, 9, 14, and 17 weeks of age. Parameters assessed in this study included offspring number, sex ratios, body weights, testis weights, seminiferous tubule (ST) profile numbers and diameters, number of vimentin-labeled Sertoli cells, and both testosterone and follicle-stimulating hormone (FSH) levels. Testicular weight/body weight ratios and the numbers and diameters of ST in maximum transverse testicular sections were statistically similar at weeks 7 and 9; however, at weeks 14 and 17, they were statistically different and displayed higher BrdU-positive Sertoli cells/Sertoli cell ratios in the DBP treatment group. Noteworthily, the serum FSH levels were higher and testicular testosterone levels were lower in the DBP treatment group. To our knowledge, the present study is the first to report that in utero DBP exposure significantly increased Sertoli cell numbers and their cellular proliferation from postpuberty to adulthood, with a significant decrease in testicular testosterone and an increase in FSH. |
| Databáze: | OpenAIRE |
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