Identification of molecular apocrine breast tumours by microarray analysis
| Autor: | Jonas Bergh, Hervé Bonnefoi, Anne-Laure Nicoulaz, Pierre Farmer, Darlene R. Goldstein, Mauro Delorenzi, Maryse Fiche, David Cameron, Gaëtan MacGrogan, Véronique Becette, Cathrin Brisken, Michèle Tubiana-Hulin, Richard Iggo, Pierre Fumoleau, Denis Larsimont, Stephan Duss |
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| Rok vydání: | 2005 |
| Předmět: |
Pathology
Cancer Research medicine.medical_specialty Microarray Receptor ErbB-2 medicine.drug_class Biopsy Androgen Receptor Positive Breast Neoplasms Biology Basal (phylogenetics) Breast cancer Internal medicine Gene expression Genetics medicine Humans Molecular Biology Oligonucleotide Array Sequence Analysis Microarray analysis techniques Apocrine Cancer Apocrine Carcinoma Androgen medicine.disease Androgen receptor Apocrine Glands Phenotype Endocrinology Receptors Estrogen Receptors Androgen Poster Presentation Cancer research Female Estrogen receptor alpha Signal Transduction |
| Zdroj: | Breast Cancer Research : BCR |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | Previous microarray studies on breast cancer identified multiple tumour classes, of which the most prominent, named luminal and basal, differ in expression of the estrogen receptor alpha gene (ER). We report here the identification of a group of breast tumours with increased androgen signalling and a 'molecular apocrine' gene expression profile. Tumour samples from 49 patients with large operable or locally advanced breast cancers were tested on Affymetrix U133A gene expression microarrays. Principal components analysis and hierarchical clustering split the tumours into three groups: basal, luminal, and a group we call molecular apocrine. All of the molecular apocrine tumours have strong apocrine features on histological examination (P = 0.0002). The molecular apocrine group is androgen receptor-positive (AR+) and contains all of the ER-negative tumours outside the basal group. Kolmogorov-Smirnov testing indicates that oestrogen signalling is most active in the luminal group, and androgen signalling is most active in the molecular apocrine group. ERBB2 amplification is more common in the molecular apocrine group than the other groups. Genes that best split the three groups were identified by the Wilcoxon test. Correlation of the average expression profile of these genes in our data with the expression profile of individual tumours in four published breast cancer studies suggest that molecular apocrine tumours represent 8–14% of tumours in these studies. Our data show that it is possible with microarray data to divide mammary tumour cells into three groups based on steroid receptor activity: luminal (ER+ AR+), basal (ER-AR-) and molecular apocrine (ER- AR+). |
| Databáze: | OpenAIRE |
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